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Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI

PURPOSE: The purpose of this study was to develop a tool for functional phenotyping of the maternal circulation in the mouse placenta. PROCEDURES: In utero macromolecular dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was performed on embryonic day 10.5 (E10.5), E13.5, and E18.5. Flu...

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Autores principales: Plaks, Vicki, Sapoznik, Stav, Berkovitz, Elina, Haffner-Krausz, Rebecca, Dekel, Nava, Harmelin, Alon, Neeman, Michal
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087877/
https://www.ncbi.nlm.nih.gov/pubmed/20686857
http://dx.doi.org/10.1007/s11307-010-0390-1
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author Plaks, Vicki
Sapoznik, Stav
Berkovitz, Elina
Haffner-Krausz, Rebecca
Dekel, Nava
Harmelin, Alon
Neeman, Michal
author_facet Plaks, Vicki
Sapoznik, Stav
Berkovitz, Elina
Haffner-Krausz, Rebecca
Dekel, Nava
Harmelin, Alon
Neeman, Michal
author_sort Plaks, Vicki
collection PubMed
description PURPOSE: The purpose of this study was to develop a tool for functional phenotyping of the maternal circulation in the mouse placenta. PROCEDURES: In utero macromolecular dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was performed on embryonic day 10.5 (E10.5), E13.5, and E18.5. Fluorescence analysis was also used for validation of the results. RESULTS: The initial rate of contrast enhancement revealed an increased maternal blood volume fraction as the pregnancy progressed. Serial imaging of E10.5 and E13.5 placentas revealed a loss of contrast enhancement due to phagocytic uptake. A key application of macromolecular DCE-MRI would be to follow mouse pregnancies during fetal and placental manipulation including embryo transfer, tetraploid complementation, and fetal resorptions. We were able to resolve strain differences in ICR outbred mice carrying both ICR and C57Bl/6J embryos and to differentiate in utero resorptions from functional placentas. CONCLUSIONS: Our results highlight the importance of the functional in utero analysis of placental vascularization in physiological phenotyping of transgenic mice and suggest MRI, particularly macromolecular DCE-MRI, as a non-invasive tool for the analysis of the placenta.
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spelling pubmed-30878772011-06-06 Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI Plaks, Vicki Sapoznik, Stav Berkovitz, Elina Haffner-Krausz, Rebecca Dekel, Nava Harmelin, Alon Neeman, Michal Mol Imaging Biol Research Article PURPOSE: The purpose of this study was to develop a tool for functional phenotyping of the maternal circulation in the mouse placenta. PROCEDURES: In utero macromolecular dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was performed on embryonic day 10.5 (E10.5), E13.5, and E18.5. Fluorescence analysis was also used for validation of the results. RESULTS: The initial rate of contrast enhancement revealed an increased maternal blood volume fraction as the pregnancy progressed. Serial imaging of E10.5 and E13.5 placentas revealed a loss of contrast enhancement due to phagocytic uptake. A key application of macromolecular DCE-MRI would be to follow mouse pregnancies during fetal and placental manipulation including embryo transfer, tetraploid complementation, and fetal resorptions. We were able to resolve strain differences in ICR outbred mice carrying both ICR and C57Bl/6J embryos and to differentiate in utero resorptions from functional placentas. CONCLUSIONS: Our results highlight the importance of the functional in utero analysis of placental vascularization in physiological phenotyping of transgenic mice and suggest MRI, particularly macromolecular DCE-MRI, as a non-invasive tool for the analysis of the placenta. Springer-Verlag 2010-08-05 2011 /pmc/articles/PMC3087877/ /pubmed/20686857 http://dx.doi.org/10.1007/s11307-010-0390-1 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Research Article
Plaks, Vicki
Sapoznik, Stav
Berkovitz, Elina
Haffner-Krausz, Rebecca
Dekel, Nava
Harmelin, Alon
Neeman, Michal
Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI
title Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI
title_full Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI
title_fullStr Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI
title_full_unstemmed Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI
title_short Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI
title_sort functional phenotyping of the maternal albumin turnover in the mouse placenta by dynamic contrast-enhanced mri
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087877/
https://www.ncbi.nlm.nih.gov/pubmed/20686857
http://dx.doi.org/10.1007/s11307-010-0390-1
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