Cargando…

Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation

Non-human primates (NHPs) are a very valuable experimental model for diabetes research studies including experimental pancreatic islet transplantation. In particular NHPs are the recipients of choice to validate pigs as possible source of pancreatic islets. The aim of this study was to quantify glyc...

Descripción completa

Detalles Bibliográficos
Autores principales: Marigliano, Marco, Casu, Anna, Bertera, Suzanne, Trucco, Massimo, Bottino, Rita
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087943/
https://www.ncbi.nlm.nih.gov/pubmed/21559266
http://dx.doi.org/10.1155/2011/965605
_version_ 1782202838008463360
author Marigliano, Marco
Casu, Anna
Bertera, Suzanne
Trucco, Massimo
Bottino, Rita
author_facet Marigliano, Marco
Casu, Anna
Bertera, Suzanne
Trucco, Massimo
Bottino, Rita
author_sort Marigliano, Marco
collection PubMed
description Non-human primates (NHPs) are a very valuable experimental model for diabetes research studies including experimental pancreatic islet transplantation. In particular NHPs are the recipients of choice to validate pigs as possible source of pancreatic islets. The aim of this study was to quantify glycated hemoglobin percentage in NHPs and to assess whether changes in values reflect the metabolic trends after diabetes induction and islet transplantation. Sera from 15 NHPs were analyzed. 9 NHPs were rendered diabetic with streptozotocin (STZ), and 3 of them received porcine islet transplants. Hemoglobin A1c (HbA1c) percentage was measured with an assay based on a latex immunoagglutination inhibition methodology. Whereas diabetes and its duration were associated with increasing HbA1c levels, postislet transplantation blood glucose normalization was paralleled by a decrease in the HbA1c percentage. Our data provide evidence that HbA1c is a useful tool to monitor glucose metabolism in NHPs.
format Text
id pubmed-3087943
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-30879432011-05-10 Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation Marigliano, Marco Casu, Anna Bertera, Suzanne Trucco, Massimo Bottino, Rita J Transplant Research Article Non-human primates (NHPs) are a very valuable experimental model for diabetes research studies including experimental pancreatic islet transplantation. In particular NHPs are the recipients of choice to validate pigs as possible source of pancreatic islets. The aim of this study was to quantify glycated hemoglobin percentage in NHPs and to assess whether changes in values reflect the metabolic trends after diabetes induction and islet transplantation. Sera from 15 NHPs were analyzed. 9 NHPs were rendered diabetic with streptozotocin (STZ), and 3 of them received porcine islet transplants. Hemoglobin A1c (HbA1c) percentage was measured with an assay based on a latex immunoagglutination inhibition methodology. Whereas diabetes and its duration were associated with increasing HbA1c levels, postislet transplantation blood glucose normalization was paralleled by a decrease in the HbA1c percentage. Our data provide evidence that HbA1c is a useful tool to monitor glucose metabolism in NHPs. Hindawi Publishing Corporation 2011 2011-04-17 /pmc/articles/PMC3087943/ /pubmed/21559266 http://dx.doi.org/10.1155/2011/965605 Text en Copyright © 2011 Marco Marigliano et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Marigliano, Marco
Casu, Anna
Bertera, Suzanne
Trucco, Massimo
Bottino, Rita
Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
title Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
title_full Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
title_fullStr Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
title_full_unstemmed Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
title_short Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool to Monitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
title_sort hemoglobin a1c percentage in nonhuman primates: a useful tool to monitor diabetes before and after porcine pancreatic islet xenotransplantation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087943/
https://www.ncbi.nlm.nih.gov/pubmed/21559266
http://dx.doi.org/10.1155/2011/965605
work_keys_str_mv AT mariglianomarco hemoglobina1cpercentageinnonhumanprimatesausefultooltomonitordiabetesbeforeandafterporcinepancreaticisletxenotransplantation
AT casuanna hemoglobina1cpercentageinnonhumanprimatesausefultooltomonitordiabetesbeforeandafterporcinepancreaticisletxenotransplantation
AT berterasuzanne hemoglobina1cpercentageinnonhumanprimatesausefultooltomonitordiabetesbeforeandafterporcinepancreaticisletxenotransplantation
AT truccomassimo hemoglobina1cpercentageinnonhumanprimatesausefultooltomonitordiabetesbeforeandafterporcinepancreaticisletxenotransplantation
AT bottinorita hemoglobina1cpercentageinnonhumanprimatesausefultooltomonitordiabetesbeforeandafterporcinepancreaticisletxenotransplantation