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Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model

Radiotherapy used in the treatment of pediatric musculoskeletal sarcomas may result in crippling defects of skeletal growth. Several radioprotective strategies have shown potential for preserving function of the irradiated epiphysis but have not been evaluated in a tumor-bearing animal model. We dev...

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Detalles Bibliográficos
Autores principales: Horton, Jason A., Strauss, Judith A., Allen, Matthew J., Damron, Timothy A.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087963/
https://www.ncbi.nlm.nih.gov/pubmed/21559211
http://dx.doi.org/10.1155/2011/815190
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author Horton, Jason A.
Strauss, Judith A.
Allen, Matthew J.
Damron, Timothy A.
author_facet Horton, Jason A.
Strauss, Judith A.
Allen, Matthew J.
Damron, Timothy A.
author_sort Horton, Jason A.
collection PubMed
description Radiotherapy used in the treatment of pediatric musculoskeletal sarcomas may result in crippling defects of skeletal growth. Several radioprotective strategies have shown potential for preserving function of the irradiated epiphysis but have not been evaluated in a tumor-bearing animal model. We developed two bioluminescent human rhabdomyosarcoma cell lines that were used to establish xenograft tumors in skeletally immature mice. Bioluminescence imaging and radiography allowed serial evaluation of tumor growth and tibial elongation following localized radiotherapy. High-dose (10 Gy) radiotherapy significantly reduced tumor growth velocity and prolonged the median survival of tumor-bearing mice but also resulted in a significant 3.3% shortening of the irradiated limb. Exposure to a lower, 2 Gy dose resulted in 4.1% decrease in limb length but did not extend survival. This new model provides a clinically relevant means to test the efficacy and safety of novel radioprotectant and radiorecovery strategies for use in this context.
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spelling pubmed-30879632011-05-10 Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model Horton, Jason A. Strauss, Judith A. Allen, Matthew J. Damron, Timothy A. Sarcoma Research Article Radiotherapy used in the treatment of pediatric musculoskeletal sarcomas may result in crippling defects of skeletal growth. Several radioprotective strategies have shown potential for preserving function of the irradiated epiphysis but have not been evaluated in a tumor-bearing animal model. We developed two bioluminescent human rhabdomyosarcoma cell lines that were used to establish xenograft tumors in skeletally immature mice. Bioluminescence imaging and radiography allowed serial evaluation of tumor growth and tibial elongation following localized radiotherapy. High-dose (10 Gy) radiotherapy significantly reduced tumor growth velocity and prolonged the median survival of tumor-bearing mice but also resulted in a significant 3.3% shortening of the irradiated limb. Exposure to a lower, 2 Gy dose resulted in 4.1% decrease in limb length but did not extend survival. This new model provides a clinically relevant means to test the efficacy and safety of novel radioprotectant and radiorecovery strategies for use in this context. Hindawi Publishing Corporation 2011 2011-04-17 /pmc/articles/PMC3087963/ /pubmed/21559211 http://dx.doi.org/10.1155/2011/815190 Text en Copyright © 2011 Jason A. Horton et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Horton, Jason A.
Strauss, Judith A.
Allen, Matthew J.
Damron, Timothy A.
Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model
title Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model
title_full Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model
title_fullStr Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model
title_full_unstemmed Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model
title_short Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model
title_sort physeal bystander effects in rhabdomyosarcoma radiotherapy: experiments in a new xenograft model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087963/
https://www.ncbi.nlm.nih.gov/pubmed/21559211
http://dx.doi.org/10.1155/2011/815190
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