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Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain
Although mammalian neurogenesis is mostly completed by the perinatal period, new neurons are continuously generated in the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Since the discovery of adult neurogenesis, many extensive studies have be...
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Formato: | Texto |
Lenguaje: | English |
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Frontiers Research Foundation
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087966/ https://www.ncbi.nlm.nih.gov/pubmed/21562606 http://dx.doi.org/10.3389/fnins.2011.00064 |
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author | Imayoshi, Itaru Sakamoto, Masayuki Kageyama, Ryoichiro |
author_facet | Imayoshi, Itaru Sakamoto, Masayuki Kageyama, Ryoichiro |
author_sort | Imayoshi, Itaru |
collection | PubMed |
description | Although mammalian neurogenesis is mostly completed by the perinatal period, new neurons are continuously generated in the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Since the discovery of adult neurogenesis, many extensive studies have been performed on various aspects of adult neurogenesis, including proliferation and fate-specification of adult neural stem cells, and the migration, maturation and synaptic integration of newly born neurons. Furthermore, recent research has shed light on the intensive contribution of adult neurogenesis to olfactory-related and hippocampus-mediated brain functions. The field of adult neurogenesis progressed tremendously thanks to technical advances that facilitate the identification and selective manipulation of newly born neurons among billions of pre-existing neurons in the adult central nervous system. In this review, we introduce recent advances in the methodologies for visualizing newly generated neurons and manipulating neurogenesis in the adult brain. Particularly, the application of site-specific recombinases and Tet inducible system in combination with transgenic or gene targeting strategy is discussed in further detail. |
format | Text |
id | pubmed-3087966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-30879662011-05-11 Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain Imayoshi, Itaru Sakamoto, Masayuki Kageyama, Ryoichiro Front Neurosci Neuroscience Although mammalian neurogenesis is mostly completed by the perinatal period, new neurons are continuously generated in the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Since the discovery of adult neurogenesis, many extensive studies have been performed on various aspects of adult neurogenesis, including proliferation and fate-specification of adult neural stem cells, and the migration, maturation and synaptic integration of newly born neurons. Furthermore, recent research has shed light on the intensive contribution of adult neurogenesis to olfactory-related and hippocampus-mediated brain functions. The field of adult neurogenesis progressed tremendously thanks to technical advances that facilitate the identification and selective manipulation of newly born neurons among billions of pre-existing neurons in the adult central nervous system. In this review, we introduce recent advances in the methodologies for visualizing newly generated neurons and manipulating neurogenesis in the adult brain. Particularly, the application of site-specific recombinases and Tet inducible system in combination with transgenic or gene targeting strategy is discussed in further detail. Frontiers Research Foundation 2011-05-02 /pmc/articles/PMC3087966/ /pubmed/21562606 http://dx.doi.org/10.3389/fnins.2011.00064 Text en Copyright © 2011 Imayoshi, Sakamoto and Kageyama. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with. |
spellingShingle | Neuroscience Imayoshi, Itaru Sakamoto, Masayuki Kageyama, Ryoichiro Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain |
title | Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain |
title_full | Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain |
title_fullStr | Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain |
title_full_unstemmed | Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain |
title_short | Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain |
title_sort | genetic methods to identify and manipulate newly born neurons in the adult brain |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087966/ https://www.ncbi.nlm.nih.gov/pubmed/21562606 http://dx.doi.org/10.3389/fnins.2011.00064 |
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