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The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida

The RNA-binding protein Hfq is recognized as an important regulatory factor in a variety of cellular processes, including stress resistance and pathogenesis. Hfq has been shown in several bacteria to interact with small regulatory RNAs and act as a post-transcriptional regulator of mRNA stability an...

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Detalles Bibliográficos
Autores principales: Chambers, Jacob R., Bender, Kelly S.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3088715/
https://www.ncbi.nlm.nih.gov/pubmed/21573133
http://dx.doi.org/10.1371/journal.pone.0019797
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author Chambers, Jacob R.
Bender, Kelly S.
author_facet Chambers, Jacob R.
Bender, Kelly S.
author_sort Chambers, Jacob R.
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description The RNA-binding protein Hfq is recognized as an important regulatory factor in a variety of cellular processes, including stress resistance and pathogenesis. Hfq has been shown in several bacteria to interact with small regulatory RNAs and act as a post-transcriptional regulator of mRNA stability and translation. Here we examined the impact of Hfq on growth, stress tolerance, and gene expression in the intracellular pathogen Francisella novicida. We present evidence of Hfq involvement in the ability of F. novicida to tolerate several cellular stresses, including heat-shock and oxidative stresses, and alterations in hfq gene expression under these conditions. Furthermore, expression of numerous genes, including several associated with virulence, is altered in a hfq mutant strain suggesting they are regulated directly or indirectly by Hfq. Strikingly, we observed a delayed entry into stationary phase and increased biofilm formation in the hfq mutant. Together, these data demonstrate a critical role for Hfq in F. novicida growth and survival.
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spelling pubmed-30887152011-05-13 The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida Chambers, Jacob R. Bender, Kelly S. PLoS One Research Article The RNA-binding protein Hfq is recognized as an important regulatory factor in a variety of cellular processes, including stress resistance and pathogenesis. Hfq has been shown in several bacteria to interact with small regulatory RNAs and act as a post-transcriptional regulator of mRNA stability and translation. Here we examined the impact of Hfq on growth, stress tolerance, and gene expression in the intracellular pathogen Francisella novicida. We present evidence of Hfq involvement in the ability of F. novicida to tolerate several cellular stresses, including heat-shock and oxidative stresses, and alterations in hfq gene expression under these conditions. Furthermore, expression of numerous genes, including several associated with virulence, is altered in a hfq mutant strain suggesting they are regulated directly or indirectly by Hfq. Strikingly, we observed a delayed entry into stationary phase and increased biofilm formation in the hfq mutant. Together, these data demonstrate a critical role for Hfq in F. novicida growth and survival. Public Library of Science 2011-05-05 /pmc/articles/PMC3088715/ /pubmed/21573133 http://dx.doi.org/10.1371/journal.pone.0019797 Text en Chambers, Bender. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chambers, Jacob R.
Bender, Kelly S.
The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida
title The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida
title_full The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida
title_fullStr The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida
title_full_unstemmed The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida
title_short The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida
title_sort rna chaperone hfq is important for growth and stress tolerance in francisella novicida
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3088715/
https://www.ncbi.nlm.nih.gov/pubmed/21573133
http://dx.doi.org/10.1371/journal.pone.0019797
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