Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells

BACKGROUND: Molecular cloning of functional immunoglobulin genes from single plasma cells is one of the most promising technologies for the rapid development of monoclonal antibody drugs. However, the proper insertion of PCR-amplified immunoglobulin genes into expression vectors remains an obstacle...

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Detalles Bibliográficos
Autores principales: Kurosawa, Nobuyuki, Yoshioka, Megumi, Isobe, Masaharu
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3088891/
https://www.ncbi.nlm.nih.gov/pubmed/21486488
http://dx.doi.org/10.1186/1472-6750-11-39

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3088891/
https://www.ncbi.nlm.nih.gov/pubmed/21486488
http://dx.doi.org/10.1186/1472-6750-11-39

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