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Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells
BACKGROUND: Molecular cloning of functional immunoglobulin genes from single plasma cells is one of the most promising technologies for the rapid development of monoclonal antibody drugs. However, the proper insertion of PCR-amplified immunoglobulin genes into expression vectors remains an obstacle...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3088891/ https://www.ncbi.nlm.nih.gov/pubmed/21486488 http://dx.doi.org/10.1186/1472-6750-11-39 |