Cargando…

Identification of human miRNA precursors that resemble box C/D snoRNAs

There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible e...

Descripción completa

Detalles Bibliográficos
Autores principales: Ono, Motoharu, Scott, Michelle S., Yamada, Kayo, Avolio, Fabio, Barton, Geoffrey J., Lamond, Angus I.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089480/
https://www.ncbi.nlm.nih.gov/pubmed/21247878
http://dx.doi.org/10.1093/nar/gkq1355
_version_ 1782203052966543360
author Ono, Motoharu
Scott, Michelle S.
Yamada, Kayo
Avolio, Fabio
Barton, Geoffrey J.
Lamond, Angus I.
author_facet Ono, Motoharu
Scott, Michelle S.
Yamada, Kayo
Avolio, Fabio
Barton, Geoffrey J.
Lamond, Angus I.
author_sort Ono, Motoharu
collection PubMed
description There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.
format Text
id pubmed-3089480
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-30894802011-05-09 Identification of human miRNA precursors that resemble box C/D snoRNAs Ono, Motoharu Scott, Michelle S. Yamada, Kayo Avolio, Fabio Barton, Geoffrey J. Lamond, Angus I. Nucleic Acids Res RNA There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs. Oxford University Press 2011-05 2011-01-18 /pmc/articles/PMC3089480/ /pubmed/21247878 http://dx.doi.org/10.1093/nar/gkq1355 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Ono, Motoharu
Scott, Michelle S.
Yamada, Kayo
Avolio, Fabio
Barton, Geoffrey J.
Lamond, Angus I.
Identification of human miRNA precursors that resemble box C/D snoRNAs
title Identification of human miRNA precursors that resemble box C/D snoRNAs
title_full Identification of human miRNA precursors that resemble box C/D snoRNAs
title_fullStr Identification of human miRNA precursors that resemble box C/D snoRNAs
title_full_unstemmed Identification of human miRNA precursors that resemble box C/D snoRNAs
title_short Identification of human miRNA precursors that resemble box C/D snoRNAs
title_sort identification of human mirna precursors that resemble box c/d snornas
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089480/
https://www.ncbi.nlm.nih.gov/pubmed/21247878
http://dx.doi.org/10.1093/nar/gkq1355
work_keys_str_mv AT onomotoharu identificationofhumanmirnaprecursorsthatresembleboxcdsnornas
AT scottmichelles identificationofhumanmirnaprecursorsthatresembleboxcdsnornas
AT yamadakayo identificationofhumanmirnaprecursorsthatresembleboxcdsnornas
AT avoliofabio identificationofhumanmirnaprecursorsthatresembleboxcdsnornas
AT bartongeoffreyj identificationofhumanmirnaprecursorsthatresembleboxcdsnornas
AT lamondangusi identificationofhumanmirnaprecursorsthatresembleboxcdsnornas