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Comparative Genomics of Cell Envelope Components in Mycobacteria

Mycobacterial cell envelope components have been a major focus of research due to their unique features that confer intrinsic resistance to antibiotics and chemicals apart from serving as a low-permeability barrier. The complex lipids secreted by Mycobacteria are known to evoke/repress host-immune r...

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Autores principales: Banerjee, Ruma, Vats, Pankaj, Dahale, Sonal, Kasibhatla, Sunitha Manjari, Joshi, Rajendra
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089613/
https://www.ncbi.nlm.nih.gov/pubmed/21573108
http://dx.doi.org/10.1371/journal.pone.0019280
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author Banerjee, Ruma
Vats, Pankaj
Dahale, Sonal
Kasibhatla, Sunitha Manjari
Joshi, Rajendra
author_facet Banerjee, Ruma
Vats, Pankaj
Dahale, Sonal
Kasibhatla, Sunitha Manjari
Joshi, Rajendra
author_sort Banerjee, Ruma
collection PubMed
description Mycobacterial cell envelope components have been a major focus of research due to their unique features that confer intrinsic resistance to antibiotics and chemicals apart from serving as a low-permeability barrier. The complex lipids secreted by Mycobacteria are known to evoke/repress host-immune response and thus contribute to its pathogenicity. This study focuses on the comparative genomics of the biosynthetic machinery of cell wall components across 21-mycobacterial genomes available in GenBank release 179.0. An insight into survival in varied environments could be attributed to its variation in the biosynthetic machinery. Gene-specific motifs like ‘DLLAQPTPAW’ of ufaA1 gene, novel functional linkages such as involvement of Rv0227c in mycolate biosynthesis; Rv2613c in LAM biosynthesis and Rv1209 in arabinogalactan peptidoglycan biosynthesis were detected in this study. These predictions correlate well with the available mutant and coexpression data from TBDB. It also helped to arrive at a minimal functional gene set for these biosynthetic pathways that complements findings using TraSH.
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spelling pubmed-30896132011-05-13 Comparative Genomics of Cell Envelope Components in Mycobacteria Banerjee, Ruma Vats, Pankaj Dahale, Sonal Kasibhatla, Sunitha Manjari Joshi, Rajendra PLoS One Research Article Mycobacterial cell envelope components have been a major focus of research due to their unique features that confer intrinsic resistance to antibiotics and chemicals apart from serving as a low-permeability barrier. The complex lipids secreted by Mycobacteria are known to evoke/repress host-immune response and thus contribute to its pathogenicity. This study focuses on the comparative genomics of the biosynthetic machinery of cell wall components across 21-mycobacterial genomes available in GenBank release 179.0. An insight into survival in varied environments could be attributed to its variation in the biosynthetic machinery. Gene-specific motifs like ‘DLLAQPTPAW’ of ufaA1 gene, novel functional linkages such as involvement of Rv0227c in mycolate biosynthesis; Rv2613c in LAM biosynthesis and Rv1209 in arabinogalactan peptidoglycan biosynthesis were detected in this study. These predictions correlate well with the available mutant and coexpression data from TBDB. It also helped to arrive at a minimal functional gene set for these biosynthetic pathways that complements findings using TraSH. Public Library of Science 2011-05-06 /pmc/articles/PMC3089613/ /pubmed/21573108 http://dx.doi.org/10.1371/journal.pone.0019280 Text en Banerjee et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Banerjee, Ruma
Vats, Pankaj
Dahale, Sonal
Kasibhatla, Sunitha Manjari
Joshi, Rajendra
Comparative Genomics of Cell Envelope Components in Mycobacteria
title Comparative Genomics of Cell Envelope Components in Mycobacteria
title_full Comparative Genomics of Cell Envelope Components in Mycobacteria
title_fullStr Comparative Genomics of Cell Envelope Components in Mycobacteria
title_full_unstemmed Comparative Genomics of Cell Envelope Components in Mycobacteria
title_short Comparative Genomics of Cell Envelope Components in Mycobacteria
title_sort comparative genomics of cell envelope components in mycobacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089613/
https://www.ncbi.nlm.nih.gov/pubmed/21573108
http://dx.doi.org/10.1371/journal.pone.0019280
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