Cargando…

Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration

BACKGROUND: Heat shock proteins are acute phase proteins that are upregulated in inflammation or following thermal stress. We analyzed the presence of the heat shock protein 70 (Hsp 70) in choroidal neovascular (CNV) membranes secondary to AMD after treatment with verteporphin photodynamic therapy (...

Descripción completa

Detalles Bibliográficos
Autores principales: Jöres, Andreas PW, Carstesen, Dörthe, Thumann, Gabriele, Walter, Peter, Weinberger, Andreas WA
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089788/
https://www.ncbi.nlm.nih.gov/pubmed/21477309
http://dx.doi.org/10.1186/1756-0500-4-115
_version_ 1782203078848544768
author Jöres, Andreas PW
Carstesen, Dörthe
Thumann, Gabriele
Walter, Peter
Weinberger, Andreas WA
author_facet Jöres, Andreas PW
Carstesen, Dörthe
Thumann, Gabriele
Walter, Peter
Weinberger, Andreas WA
author_sort Jöres, Andreas PW
collection PubMed
description BACKGROUND: Heat shock proteins are acute phase proteins that are upregulated in inflammation or following thermal stress. We analyzed the presence of the heat shock protein 70 (Hsp 70) in choroidal neovascular (CNV) membranes secondary to AMD after treatment with verteporphin photodynamic therapy (PDT) or transpupillary thermo therapy (TTT) to determine whether treatment correlated with the presence of Hsp70. RESULTS: CNV membranes were removed by pars plana vitrectomy (ppV) and subretinal extraction. The membranes were analysed by light microscopy and the presence of Hsp 70 was examined using histochemistry. HeLa Cells served as controls. Of the 14 membranes analysed 11 were Hsp70 positive and 3 negative. In the no pre-treatment group of 8 membranes 6 were Hsp70 positive and 2 negative; in the PTD group all 4 membranes were positive and in the TTT group 1 membrane was positive and 1 membrane was negative for Hsp70. CONCLUSION: Hsp70 is present in the most CNV membranes secondary to AMD. Pre-treatment of the membrane with PTD or TTT does not appear to influence the expression of Hsp70.
format Text
id pubmed-3089788
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30897882011-05-08 Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration Jöres, Andreas PW Carstesen, Dörthe Thumann, Gabriele Walter, Peter Weinberger, Andreas WA BMC Res Notes Research Article BACKGROUND: Heat shock proteins are acute phase proteins that are upregulated in inflammation or following thermal stress. We analyzed the presence of the heat shock protein 70 (Hsp 70) in choroidal neovascular (CNV) membranes secondary to AMD after treatment with verteporphin photodynamic therapy (PDT) or transpupillary thermo therapy (TTT) to determine whether treatment correlated with the presence of Hsp70. RESULTS: CNV membranes were removed by pars plana vitrectomy (ppV) and subretinal extraction. The membranes were analysed by light microscopy and the presence of Hsp 70 was examined using histochemistry. HeLa Cells served as controls. Of the 14 membranes analysed 11 were Hsp70 positive and 3 negative. In the no pre-treatment group of 8 membranes 6 were Hsp70 positive and 2 negative; in the PTD group all 4 membranes were positive and in the TTT group 1 membrane was positive and 1 membrane was negative for Hsp70. CONCLUSION: Hsp70 is present in the most CNV membranes secondary to AMD. Pre-treatment of the membrane with PTD or TTT does not appear to influence the expression of Hsp70. BioMed Central 2011-04-08 /pmc/articles/PMC3089788/ /pubmed/21477309 http://dx.doi.org/10.1186/1756-0500-4-115 Text en Copyright ©2011 Jöres et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jöres, Andreas PW
Carstesen, Dörthe
Thumann, Gabriele
Walter, Peter
Weinberger, Andreas WA
Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration
title Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration
title_full Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration
title_fullStr Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration
title_full_unstemmed Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration
title_short Detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration
title_sort detection of heat shock protein 70 in choroidal neovascular membranes secondary to age related macular degeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089788/
https://www.ncbi.nlm.nih.gov/pubmed/21477309
http://dx.doi.org/10.1186/1756-0500-4-115
work_keys_str_mv AT joresandreaspw detectionofheatshockprotein70inchoroidalneovascularmembranessecondarytoagerelatedmaculardegeneration
AT carstesendorthe detectionofheatshockprotein70inchoroidalneovascularmembranessecondarytoagerelatedmaculardegeneration
AT thumanngabriele detectionofheatshockprotein70inchoroidalneovascularmembranessecondarytoagerelatedmaculardegeneration
AT walterpeter detectionofheatshockprotein70inchoroidalneovascularmembranessecondarytoagerelatedmaculardegeneration
AT weinbergerandreaswa detectionofheatshockprotein70inchoroidalneovascularmembranessecondarytoagerelatedmaculardegeneration