Cargando…

A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa

Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (P...

Descripción completa

Detalles Bibliográficos
Autores principales: Glorieux, Sarah, Favoreel, Herman W, Steukers, Lennert, Vandekerckhove, Annelies P, Nauwynck, Hans J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089791/
https://www.ncbi.nlm.nih.gov/pubmed/21492440
http://dx.doi.org/10.1186/1297-9716-42-58
_version_ 1782203079536410624
author Glorieux, Sarah
Favoreel, Herman W
Steukers, Lennert
Vandekerckhove, Annelies P
Nauwynck, Hans J
author_facet Glorieux, Sarah
Favoreel, Herman W
Steukers, Lennert
Vandekerckhove, Annelies P
Nauwynck, Hans J
author_sort Glorieux, Sarah
collection PubMed
description Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane.
format Text
id pubmed-3089791
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30897912011-05-08 A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa Glorieux, Sarah Favoreel, Herman W Steukers, Lennert Vandekerckhove, Annelies P Nauwynck, Hans J Vet Res Short Report Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane. BioMed Central 2011 2011-04-14 /pmc/articles/PMC3089791/ /pubmed/21492440 http://dx.doi.org/10.1186/1297-9716-42-58 Text en Copyright ©2011 Glorieux et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Glorieux, Sarah
Favoreel, Herman W
Steukers, Lennert
Vandekerckhove, Annelies P
Nauwynck, Hans J
A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
title A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
title_full A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
title_fullStr A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
title_full_unstemmed A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
title_short A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
title_sort trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089791/
https://www.ncbi.nlm.nih.gov/pubmed/21492440
http://dx.doi.org/10.1186/1297-9716-42-58
work_keys_str_mv AT glorieuxsarah atrypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT favoreelhermanw atrypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT steukerslennert atrypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT vandekerckhoveanneliesp atrypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT nauwynckhansj atrypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT glorieuxsarah trypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT favoreelhermanw trypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT steukerslennert trypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT vandekerckhoveanneliesp trypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa
AT nauwynckhansj trypsinlikeserineproteaseisinvolvedinpseudorabiesvirusinvasionthroughthebasementmembranebarrierofporcinenasalrespiratorymucosa