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Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction

PURPOSE: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of...

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Autores principales: Li, Yefei, Yao, Yuyu, Sheng, Zulong, Yang, Yanxiaoxiao, Ma, Genshan
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090278/
https://www.ncbi.nlm.nih.gov/pubmed/21589649
http://dx.doi.org/10.2147/IJN.S17611
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author Li, Yefei
Yao, Yuyu
Sheng, Zulong
Yang, Yanxiaoxiao
Ma, Genshan
author_facet Li, Yefei
Yao, Yuyu
Sheng, Zulong
Yang, Yanxiaoxiao
Ma, Genshan
author_sort Li, Yefei
collection PubMed
description PURPOSE: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of different delivery modes of mesenchymal stem cells on cell retention time and cardiac function after myocardial infarction (MI). METHODS: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles and 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for noninvasive cell tracking in a rat MI model. Rats underwent coronary artery ligation and were randomized into three experimental groups: intravenous (IV), intramyocardial (IM), and a control group. The first two groups referred to the route of delivery of the transplanted dual-labeled MSCs; whereas the control group was given an IV injection of serum-free medium one day post-MI. Cellular engraftment was determined 1 day and 7 days post cell delivery by measuring the iron and optical signals in explanted organs. Prussian blue staining and fluorescent microscopy were performed on histological sections for iron and DiD, respectively. Cardiac function was measured by echocardiography on day 7. RESULTS: The cardiac function of the IM group increased significantly compared to the IV and control groups at day 7. In the IM group, labeled cells were visualized in the infracted heart by serial MRI, and the intensity by OI was significantly higher on day 1. In the IV group, the heart signals were significantly attenuated by dual-modal tracking at two time points, but the lung signals in OI were significantly stronger than the IM group at both time points. CONCLUSION: IM injection of MSCs increased cell engraftment within infarcted hearts and improved cardiac function after MI. However, IV infusion has a low efficacy due to the cell trapping in the lung. Therefore, direct injection may provide an advantage over IV, with regard to retention of stem cells and protection of cardiac function.
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spelling pubmed-30902782011-05-17 Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction Li, Yefei Yao, Yuyu Sheng, Zulong Yang, Yanxiaoxiao Ma, Genshan Int J Nanomedicine Original Research PURPOSE: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of different delivery modes of mesenchymal stem cells on cell retention time and cardiac function after myocardial infarction (MI). METHODS: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles and 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for noninvasive cell tracking in a rat MI model. Rats underwent coronary artery ligation and were randomized into three experimental groups: intravenous (IV), intramyocardial (IM), and a control group. The first two groups referred to the route of delivery of the transplanted dual-labeled MSCs; whereas the control group was given an IV injection of serum-free medium one day post-MI. Cellular engraftment was determined 1 day and 7 days post cell delivery by measuring the iron and optical signals in explanted organs. Prussian blue staining and fluorescent microscopy were performed on histological sections for iron and DiD, respectively. Cardiac function was measured by echocardiography on day 7. RESULTS: The cardiac function of the IM group increased significantly compared to the IV and control groups at day 7. In the IM group, labeled cells were visualized in the infracted heart by serial MRI, and the intensity by OI was significantly higher on day 1. In the IV group, the heart signals were significantly attenuated by dual-modal tracking at two time points, but the lung signals in OI were significantly stronger than the IM group at both time points. CONCLUSION: IM injection of MSCs increased cell engraftment within infarcted hearts and improved cardiac function after MI. However, IV infusion has a low efficacy due to the cell trapping in the lung. Therefore, direct injection may provide an advantage over IV, with regard to retention of stem cells and protection of cardiac function. Dove Medical Press 2011 2011-04-19 /pmc/articles/PMC3090278/ /pubmed/21589649 http://dx.doi.org/10.2147/IJN.S17611 Text en © 2011 Li et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Li, Yefei
Yao, Yuyu
Sheng, Zulong
Yang, Yanxiaoxiao
Ma, Genshan
Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_full Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_fullStr Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_full_unstemmed Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_short Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_sort dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090278/
https://www.ncbi.nlm.nih.gov/pubmed/21589649
http://dx.doi.org/10.2147/IJN.S17611
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