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Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
PURPOSE: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of...
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Formato: | Texto |
Lenguaje: | English |
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Dove Medical Press
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090278/ https://www.ncbi.nlm.nih.gov/pubmed/21589649 http://dx.doi.org/10.2147/IJN.S17611 |
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author | Li, Yefei Yao, Yuyu Sheng, Zulong Yang, Yanxiaoxiao Ma, Genshan |
author_facet | Li, Yefei Yao, Yuyu Sheng, Zulong Yang, Yanxiaoxiao Ma, Genshan |
author_sort | Li, Yefei |
collection | PubMed |
description | PURPOSE: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of different delivery modes of mesenchymal stem cells on cell retention time and cardiac function after myocardial infarction (MI). METHODS: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles and 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for noninvasive cell tracking in a rat MI model. Rats underwent coronary artery ligation and were randomized into three experimental groups: intravenous (IV), intramyocardial (IM), and a control group. The first two groups referred to the route of delivery of the transplanted dual-labeled MSCs; whereas the control group was given an IV injection of serum-free medium one day post-MI. Cellular engraftment was determined 1 day and 7 days post cell delivery by measuring the iron and optical signals in explanted organs. Prussian blue staining and fluorescent microscopy were performed on histological sections for iron and DiD, respectively. Cardiac function was measured by echocardiography on day 7. RESULTS: The cardiac function of the IM group increased significantly compared to the IV and control groups at day 7. In the IM group, labeled cells were visualized in the infracted heart by serial MRI, and the intensity by OI was significantly higher on day 1. In the IV group, the heart signals were significantly attenuated by dual-modal tracking at two time points, but the lung signals in OI were significantly stronger than the IM group at both time points. CONCLUSION: IM injection of MSCs increased cell engraftment within infarcted hearts and improved cardiac function after MI. However, IV infusion has a low efficacy due to the cell trapping in the lung. Therefore, direct injection may provide an advantage over IV, with regard to retention of stem cells and protection of cardiac function. |
format | Text |
id | pubmed-3090278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30902782011-05-17 Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction Li, Yefei Yao, Yuyu Sheng, Zulong Yang, Yanxiaoxiao Ma, Genshan Int J Nanomedicine Original Research PURPOSE: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of different delivery modes of mesenchymal stem cells on cell retention time and cardiac function after myocardial infarction (MI). METHODS: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles and 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for noninvasive cell tracking in a rat MI model. Rats underwent coronary artery ligation and were randomized into three experimental groups: intravenous (IV), intramyocardial (IM), and a control group. The first two groups referred to the route of delivery of the transplanted dual-labeled MSCs; whereas the control group was given an IV injection of serum-free medium one day post-MI. Cellular engraftment was determined 1 day and 7 days post cell delivery by measuring the iron and optical signals in explanted organs. Prussian blue staining and fluorescent microscopy were performed on histological sections for iron and DiD, respectively. Cardiac function was measured by echocardiography on day 7. RESULTS: The cardiac function of the IM group increased significantly compared to the IV and control groups at day 7. In the IM group, labeled cells were visualized in the infracted heart by serial MRI, and the intensity by OI was significantly higher on day 1. In the IV group, the heart signals were significantly attenuated by dual-modal tracking at two time points, but the lung signals in OI were significantly stronger than the IM group at both time points. CONCLUSION: IM injection of MSCs increased cell engraftment within infarcted hearts and improved cardiac function after MI. However, IV infusion has a low efficacy due to the cell trapping in the lung. Therefore, direct injection may provide an advantage over IV, with regard to retention of stem cells and protection of cardiac function. Dove Medical Press 2011 2011-04-19 /pmc/articles/PMC3090278/ /pubmed/21589649 http://dx.doi.org/10.2147/IJN.S17611 Text en © 2011 Li et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Li, Yefei Yao, Yuyu Sheng, Zulong Yang, Yanxiaoxiao Ma, Genshan Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction |
title | Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction |
title_full | Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction |
title_fullStr | Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction |
title_full_unstemmed | Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction |
title_short | Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction |
title_sort | dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090278/ https://www.ncbi.nlm.nih.gov/pubmed/21589649 http://dx.doi.org/10.2147/IJN.S17611 |
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