Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity

BACKGROUND: With the aim of discovering new natural active extracts against malaria parasites, Icacina senegalensis was selected after an ethnopharmacological survey conducted on plants used in traditional malaria treatment in Senegal. METHODS: Different concentrations of the plant extract and fract...

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Autores principales: Sarr, Serigne O, Perrotey, Sylvie, Fall, Ibrahima, Ennahar, Saïd, Zhao, Minjie, Diop, Yérim M, Candolfi, Ermanno, Marchioni, Eric
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090372/
https://www.ncbi.nlm.nih.gov/pubmed/21481272
http://dx.doi.org/10.1186/1475-2875-10-85
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author Sarr, Serigne O
Perrotey, Sylvie
Fall, Ibrahima
Ennahar, Saïd
Zhao, Minjie
Diop, Yérim M
Candolfi, Ermanno
Marchioni, Eric
author_facet Sarr, Serigne O
Perrotey, Sylvie
Fall, Ibrahima
Ennahar, Saïd
Zhao, Minjie
Diop, Yérim M
Candolfi, Ermanno
Marchioni, Eric
author_sort Sarr, Serigne O
collection PubMed
description BACKGROUND: With the aim of discovering new natural active extracts against malaria parasites, Icacina senegalensis was selected after an ethnopharmacological survey conducted on plants used in traditional malaria treatment in Senegal. METHODS: Different concentrations of the plant extract and fractions were tested on synchronized Plasmodium falciparum cultures at the ring stage using the parasite lactate dehydrogenase assay. Their haemolytic activity and in vitro cytoxicity were evaluated. The chromatographic profiles of active fractions were also established. RESULTS: The plant extract and fractions revealed anti-plasmodial activity (IC(50 )< 5 μg/mL) with no toxicity (Selectivity indexes >10). The dichloromethane fraction showed stronger anti-plasmodial activity than the total extract. CONCLUSION: Anti-plasmodial activity and toxicity of I. senegalensis are reported for the first time and showed promising results in malaria field research.
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spelling pubmed-30903722011-05-10 Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity Sarr, Serigne O Perrotey, Sylvie Fall, Ibrahima Ennahar, Saïd Zhao, Minjie Diop, Yérim M Candolfi, Ermanno Marchioni, Eric Malar J Research BACKGROUND: With the aim of discovering new natural active extracts against malaria parasites, Icacina senegalensis was selected after an ethnopharmacological survey conducted on plants used in traditional malaria treatment in Senegal. METHODS: Different concentrations of the plant extract and fractions were tested on synchronized Plasmodium falciparum cultures at the ring stage using the parasite lactate dehydrogenase assay. Their haemolytic activity and in vitro cytoxicity were evaluated. The chromatographic profiles of active fractions were also established. RESULTS: The plant extract and fractions revealed anti-plasmodial activity (IC(50 )< 5 μg/mL) with no toxicity (Selectivity indexes >10). The dichloromethane fraction showed stronger anti-plasmodial activity than the total extract. CONCLUSION: Anti-plasmodial activity and toxicity of I. senegalensis are reported for the first time and showed promising results in malaria field research. BioMed Central 2011-04-11 /pmc/articles/PMC3090372/ /pubmed/21481272 http://dx.doi.org/10.1186/1475-2875-10-85 Text en Copyright ©2011 Sarr et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Sarr, Serigne O
Perrotey, Sylvie
Fall, Ibrahima
Ennahar, Saïd
Zhao, Minjie
Diop, Yérim M
Candolfi, Ermanno
Marchioni, Eric
Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity
title Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity
title_full Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity
title_fullStr Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity
title_full_unstemmed Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity
title_short Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity
title_sort icacina senegalensis (icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro plasmodium falciparum growth without host cell toxicity
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090372/
https://www.ncbi.nlm.nih.gov/pubmed/21481272
http://dx.doi.org/10.1186/1475-2875-10-85
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