Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung

It remains unknown whether tobacco smoke induces DNA hypermethylation as an early event in carcinogenesis or as a late event, specific to overt cancer tissue. Using MethyLight assays, we analyzed 316 lung tissue samples from 151 cancer-free subjects (121 ever-smokers and 30 never-smokers) for hyperm...

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Autores principales: Salskov, Alexander, Hawes, Stephen E., Stern, Joshua E., Feng, Qinghua, Jordan, C. Diana, Wiens, Linda, Rasey, Janet, Lu, Hiep, Kiviat, Nancy B., Vesselle, Hubert
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090638/
https://www.ncbi.nlm.nih.gov/pubmed/21577262
http://dx.doi.org/10.1155/2011/950140
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author Salskov, Alexander
Hawes, Stephen E.
Stern, Joshua E.
Feng, Qinghua
Jordan, C. Diana
Wiens, Linda
Rasey, Janet
Lu, Hiep
Kiviat, Nancy B.
Vesselle, Hubert
author_facet Salskov, Alexander
Hawes, Stephen E.
Stern, Joshua E.
Feng, Qinghua
Jordan, C. Diana
Wiens, Linda
Rasey, Janet
Lu, Hiep
Kiviat, Nancy B.
Vesselle, Hubert
author_sort Salskov, Alexander
collection PubMed
description It remains unknown whether tobacco smoke induces DNA hypermethylation as an early event in carcinogenesis or as a late event, specific to overt cancer tissue. Using MethyLight assays, we analyzed 316 lung tissue samples from 151 cancer-free subjects (121 ever-smokers and 30 never-smokers) for hypermethylation of 19 genes previously observed to be hypermethylated in nonsmall cell lung cancers. Only APC (39%), CCND2 (21%), CDH1 (7%), and RARB (4%) were hypermethylated in >2% of these cancer-free subjects. CCND2 was hypermethylated more frequently in ever-smokers (26%) than in never-smokers (3%). CCND2 hypermethylation was also associated with increased age and upper lobe sample location. APC was frequently hypermethylated in both ever-smokers (41%) and never-smokers (30%). BVES, CDH13, CDKN2A (p16), CDKN2B, DAPK1, IGFBP3, IGSF4, KCNH5, KCNH8, MGMT, OPCML, PCSK6, RASSF1, RUNX, and TMS1 were rarely hypermethylated (<2%) in all subjects. Hypermethylation of CCND2 may reflect a smoking-induced precancerous change in the lung.
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spelling pubmed-30906382011-05-16 Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung Salskov, Alexander Hawes, Stephen E. Stern, Joshua E. Feng, Qinghua Jordan, C. Diana Wiens, Linda Rasey, Janet Lu, Hiep Kiviat, Nancy B. Vesselle, Hubert J Oncol Research Article It remains unknown whether tobacco smoke induces DNA hypermethylation as an early event in carcinogenesis or as a late event, specific to overt cancer tissue. Using MethyLight assays, we analyzed 316 lung tissue samples from 151 cancer-free subjects (121 ever-smokers and 30 never-smokers) for hypermethylation of 19 genes previously observed to be hypermethylated in nonsmall cell lung cancers. Only APC (39%), CCND2 (21%), CDH1 (7%), and RARB (4%) were hypermethylated in >2% of these cancer-free subjects. CCND2 was hypermethylated more frequently in ever-smokers (26%) than in never-smokers (3%). CCND2 hypermethylation was also associated with increased age and upper lobe sample location. APC was frequently hypermethylated in both ever-smokers (41%) and never-smokers (30%). BVES, CDH13, CDKN2A (p16), CDKN2B, DAPK1, IGFBP3, IGSF4, KCNH5, KCNH8, MGMT, OPCML, PCSK6, RASSF1, RUNX, and TMS1 were rarely hypermethylated (<2%) in all subjects. Hypermethylation of CCND2 may reflect a smoking-induced precancerous change in the lung. Hindawi Publishing Corporation 2011 2011-03-28 /pmc/articles/PMC3090638/ /pubmed/21577262 http://dx.doi.org/10.1155/2011/950140 Text en Copyright © 2011 Alexander Salskov et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Salskov, Alexander
Hawes, Stephen E.
Stern, Joshua E.
Feng, Qinghua
Jordan, C. Diana
Wiens, Linda
Rasey, Janet
Lu, Hiep
Kiviat, Nancy B.
Vesselle, Hubert
Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung
title Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung
title_full Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung
title_fullStr Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung
title_full_unstemmed Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung
title_short Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung
title_sort hypermethylation of ccnd2 may reflect a smoking-induced precancerous change in the lung
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090638/
https://www.ncbi.nlm.nih.gov/pubmed/21577262
http://dx.doi.org/10.1155/2011/950140
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