A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready li...

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Detalles Bibliográficos
Autores principales: Fisher, Sheila, Barry, Andrew, Abreu, Justin, Minie, Brian, Nolan, Jillian, Delorey, Toni M, Young, Geneva, Fennell, Timothy J, Allen, Alexander, Ambrogio, Lauren, Berlin, Aaron M, Blumenstiel, Brendan, Cibulskis, Kristian, Friedrich, Dennis, Johnson, Ryan, Juhn, Frank, Reilly, Brian, Shammas, Ramy, Stalker, John, Sykes, Sean M, Thompson, Jon, Walsh, John, Zimmer, Andrew, Zwirko, Zac, Gabriel, Stacey, Nicol, Robert, Nusbaum, Chad
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091298/
https://www.ncbi.nlm.nih.gov/pubmed/21205303
http://dx.doi.org/10.1186/gb-2011-12-1-r1
Descripción
Sumario:Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

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