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A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready li...

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Autores principales: Fisher, Sheila, Barry, Andrew, Abreu, Justin, Minie, Brian, Nolan, Jillian, Delorey, Toni M, Young, Geneva, Fennell, Timothy J, Allen, Alexander, Ambrogio, Lauren, Berlin, Aaron M, Blumenstiel, Brendan, Cibulskis, Kristian, Friedrich, Dennis, Johnson, Ryan, Juhn, Frank, Reilly, Brian, Shammas, Ramy, Stalker, John, Sykes, Sean M, Thompson, Jon, Walsh, John, Zimmer, Andrew, Zwirko, Zac, Gabriel, Stacey, Nicol, Robert, Nusbaum, Chad
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091298/
https://www.ncbi.nlm.nih.gov/pubmed/21205303
http://dx.doi.org/10.1186/gb-2011-12-1-r1
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author Fisher, Sheila
Barry, Andrew
Abreu, Justin
Minie, Brian
Nolan, Jillian
Delorey, Toni M
Young, Geneva
Fennell, Timothy J
Allen, Alexander
Ambrogio, Lauren
Berlin, Aaron M
Blumenstiel, Brendan
Cibulskis, Kristian
Friedrich, Dennis
Johnson, Ryan
Juhn, Frank
Reilly, Brian
Shammas, Ramy
Stalker, John
Sykes, Sean M
Thompson, Jon
Walsh, John
Zimmer, Andrew
Zwirko, Zac
Gabriel, Stacey
Nicol, Robert
Nusbaum, Chad
author_facet Fisher, Sheila
Barry, Andrew
Abreu, Justin
Minie, Brian
Nolan, Jillian
Delorey, Toni M
Young, Geneva
Fennell, Timothy J
Allen, Alexander
Ambrogio, Lauren
Berlin, Aaron M
Blumenstiel, Brendan
Cibulskis, Kristian
Friedrich, Dennis
Johnson, Ryan
Juhn, Frank
Reilly, Brian
Shammas, Ramy
Stalker, John
Sykes, Sean M
Thompson, Jon
Walsh, John
Zimmer, Andrew
Zwirko, Zac
Gabriel, Stacey
Nicol, Robert
Nusbaum, Chad
author_sort Fisher, Sheila
collection PubMed
description Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
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spelling pubmed-30912982011-05-11 A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries Fisher, Sheila Barry, Andrew Abreu, Justin Minie, Brian Nolan, Jillian Delorey, Toni M Young, Geneva Fennell, Timothy J Allen, Alexander Ambrogio, Lauren Berlin, Aaron M Blumenstiel, Brendan Cibulskis, Kristian Friedrich, Dennis Johnson, Ryan Juhn, Frank Reilly, Brian Shammas, Ramy Stalker, John Sykes, Sean M Thompson, Jon Walsh, John Zimmer, Andrew Zwirko, Zac Gabriel, Stacey Nicol, Robert Nusbaum, Chad Genome Biol Method Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol. BioMed Central 2011 2011-01-04 /pmc/articles/PMC3091298/ /pubmed/21205303 http://dx.doi.org/10.1186/gb-2011-12-1-r1 Text en Copyright ©2011 Fisher et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Fisher, Sheila
Barry, Andrew
Abreu, Justin
Minie, Brian
Nolan, Jillian
Delorey, Toni M
Young, Geneva
Fennell, Timothy J
Allen, Alexander
Ambrogio, Lauren
Berlin, Aaron M
Blumenstiel, Brendan
Cibulskis, Kristian
Friedrich, Dennis
Johnson, Ryan
Juhn, Frank
Reilly, Brian
Shammas, Ramy
Stalker, John
Sykes, Sean M
Thompson, Jon
Walsh, John
Zimmer, Andrew
Zwirko, Zac
Gabriel, Stacey
Nicol, Robert
Nusbaum, Chad
A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
title A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
title_full A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
title_fullStr A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
title_full_unstemmed A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
title_short A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
title_sort scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091298/
https://www.ncbi.nlm.nih.gov/pubmed/21205303
http://dx.doi.org/10.1186/gb-2011-12-1-r1
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