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A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready li...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091298/ https://www.ncbi.nlm.nih.gov/pubmed/21205303 http://dx.doi.org/10.1186/gb-2011-12-1-r1 |
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author | Fisher, Sheila Barry, Andrew Abreu, Justin Minie, Brian Nolan, Jillian Delorey, Toni M Young, Geneva Fennell, Timothy J Allen, Alexander Ambrogio, Lauren Berlin, Aaron M Blumenstiel, Brendan Cibulskis, Kristian Friedrich, Dennis Johnson, Ryan Juhn, Frank Reilly, Brian Shammas, Ramy Stalker, John Sykes, Sean M Thompson, Jon Walsh, John Zimmer, Andrew Zwirko, Zac Gabriel, Stacey Nicol, Robert Nusbaum, Chad |
author_facet | Fisher, Sheila Barry, Andrew Abreu, Justin Minie, Brian Nolan, Jillian Delorey, Toni M Young, Geneva Fennell, Timothy J Allen, Alexander Ambrogio, Lauren Berlin, Aaron M Blumenstiel, Brendan Cibulskis, Kristian Friedrich, Dennis Johnson, Ryan Juhn, Frank Reilly, Brian Shammas, Ramy Stalker, John Sykes, Sean M Thompson, Jon Walsh, John Zimmer, Andrew Zwirko, Zac Gabriel, Stacey Nicol, Robert Nusbaum, Chad |
author_sort | Fisher, Sheila |
collection | PubMed |
description | Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol. |
format | Text |
id | pubmed-3091298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30912982011-05-11 A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries Fisher, Sheila Barry, Andrew Abreu, Justin Minie, Brian Nolan, Jillian Delorey, Toni M Young, Geneva Fennell, Timothy J Allen, Alexander Ambrogio, Lauren Berlin, Aaron M Blumenstiel, Brendan Cibulskis, Kristian Friedrich, Dennis Johnson, Ryan Juhn, Frank Reilly, Brian Shammas, Ramy Stalker, John Sykes, Sean M Thompson, Jon Walsh, John Zimmer, Andrew Zwirko, Zac Gabriel, Stacey Nicol, Robert Nusbaum, Chad Genome Biol Method Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol. BioMed Central 2011 2011-01-04 /pmc/articles/PMC3091298/ /pubmed/21205303 http://dx.doi.org/10.1186/gb-2011-12-1-r1 Text en Copyright ©2011 Fisher et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Method Fisher, Sheila Barry, Andrew Abreu, Justin Minie, Brian Nolan, Jillian Delorey, Toni M Young, Geneva Fennell, Timothy J Allen, Alexander Ambrogio, Lauren Berlin, Aaron M Blumenstiel, Brendan Cibulskis, Kristian Friedrich, Dennis Johnson, Ryan Juhn, Frank Reilly, Brian Shammas, Ramy Stalker, John Sykes, Sean M Thompson, Jon Walsh, John Zimmer, Andrew Zwirko, Zac Gabriel, Stacey Nicol, Robert Nusbaum, Chad A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
title | A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
title_full | A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
title_fullStr | A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
title_full_unstemmed | A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
title_short | A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
title_sort | scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
topic | Method |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091298/ https://www.ncbi.nlm.nih.gov/pubmed/21205303 http://dx.doi.org/10.1186/gb-2011-12-1-r1 |
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