Regulation of gene expression by FSP27 in white and brown adipose tissue

BACKGROUND: Brown and white adipose tissues (BAT and WAT) play critical roles in controlling energy homeostasis and in the development of obesity and diabetes. The mouse Fat-Specific protein 27 (FSP27), a member of the cell death-inducing DFF45-like effector (CIDE) family, is expressed in both BAT a...

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Autores principales: Li, De, Zhang, Yinxin, Xu, Li, Zhou, Linkang, Wang, Yue, Xue, Bofu, Wen, Zilong, Li, Peng, Sang, Jianli
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091643/
https://www.ncbi.nlm.nih.gov/pubmed/20649970
http://dx.doi.org/10.1186/1471-2164-11-446
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author Li, De
Zhang, Yinxin
Xu, Li
Zhou, Linkang
Wang, Yue
Xue, Bofu
Wen, Zilong
Li, Peng
Sang, Jianli
author_facet Li, De
Zhang, Yinxin
Xu, Li
Zhou, Linkang
Wang, Yue
Xue, Bofu
Wen, Zilong
Li, Peng
Sang, Jianli
author_sort Li, De
collection PubMed
description BACKGROUND: Brown and white adipose tissues (BAT and WAT) play critical roles in controlling energy homeostasis and in the development of obesity and diabetes. The mouse Fat-Specific protein 27 (FSP27), a member of the cell death-inducing DFF45-like effector (CIDE) family, is expressed in both BAT and WAT and is associated with lipid droplets. Over-expression of FSP27 promotes lipid storage, whereas FSP27 deficient mice have improved insulin sensitivity and are resistant to diet-induced obesity. In addition, FSP27-deficient white adipocytes have reduced lipid storage, smaller lipid droplets, increased mitochondrial activity and a higher expression of several BAT-selective genes. To elucidate the molecular mechanism by which FSP27 controls lipid storage and gene expression in WAT and BAT, we systematically analyzed the gene expression profile of FSP27-deficient WAT by microarray analysis and compared the expression levels of a specific set of genes in WAT and BAT by semi-quantitative real-time PCR analysis. RESULTS: BAT-selective genes were significantly up-regulated, whereas WAT-selective genes were down-regulated in the WAT of FSP27-deficient mice. The expression of the BAT-selective genes was also dramatically up-regulated in the WAT of leptin/FSP27 double deficient mice. In addition, the expression levels of genes involved in multiple metabolic pathways, including oxidative phosphorylation, the TCA cycle, fatty acid synthesis and fatty acid oxidation, were increased in the FSP27-deficient WAT. In contrast, the expression levels for genes involved in extracellular matrix remodeling, the classic complement pathway and TGF-β signaling were down-regulated in the FSP27-deficient WAT. Most importantly, the expression levels of regulatory factors that determine BAT identity, such as CEBPα/β, PRDM16 and major components of the cAMP pathway, were markedly up-regulated in the WAT of FSP27-deficient mice. The expression levels of these regulatory factors were also up-regulated in leptin/FSP27 double deficient mice. Interestingly, distinct gene expression profiles were observed in the BAT of FSP27-deficient mice. Taken together, these data suggest that the WAT of FSP27-deficient mice have a gene expression profile similar to that of BAT. CONCLUSIONS: FSP27 acts as a molecular determinant that controls gene expression for a diversity of metabolic and signaling pathways and, in particular, the expression of regulatory factors, including CEBPα/β, PRDM16 and components of the cAMP signaling pathway, that control the identity of WAT and BAT.
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spelling pubmed-30916432011-05-11 Regulation of gene expression by FSP27 in white and brown adipose tissue Li, De Zhang, Yinxin Xu, Li Zhou, Linkang Wang, Yue Xue, Bofu Wen, Zilong Li, Peng Sang, Jianli BMC Genomics Research Article BACKGROUND: Brown and white adipose tissues (BAT and WAT) play critical roles in controlling energy homeostasis and in the development of obesity and diabetes. The mouse Fat-Specific protein 27 (FSP27), a member of the cell death-inducing DFF45-like effector (CIDE) family, is expressed in both BAT and WAT and is associated with lipid droplets. Over-expression of FSP27 promotes lipid storage, whereas FSP27 deficient mice have improved insulin sensitivity and are resistant to diet-induced obesity. In addition, FSP27-deficient white adipocytes have reduced lipid storage, smaller lipid droplets, increased mitochondrial activity and a higher expression of several BAT-selective genes. To elucidate the molecular mechanism by which FSP27 controls lipid storage and gene expression in WAT and BAT, we systematically analyzed the gene expression profile of FSP27-deficient WAT by microarray analysis and compared the expression levels of a specific set of genes in WAT and BAT by semi-quantitative real-time PCR analysis. RESULTS: BAT-selective genes were significantly up-regulated, whereas WAT-selective genes were down-regulated in the WAT of FSP27-deficient mice. The expression of the BAT-selective genes was also dramatically up-regulated in the WAT of leptin/FSP27 double deficient mice. In addition, the expression levels of genes involved in multiple metabolic pathways, including oxidative phosphorylation, the TCA cycle, fatty acid synthesis and fatty acid oxidation, were increased in the FSP27-deficient WAT. In contrast, the expression levels for genes involved in extracellular matrix remodeling, the classic complement pathway and TGF-β signaling were down-regulated in the FSP27-deficient WAT. Most importantly, the expression levels of regulatory factors that determine BAT identity, such as CEBPα/β, PRDM16 and major components of the cAMP pathway, were markedly up-regulated in the WAT of FSP27-deficient mice. The expression levels of these regulatory factors were also up-regulated in leptin/FSP27 double deficient mice. Interestingly, distinct gene expression profiles were observed in the BAT of FSP27-deficient mice. Taken together, these data suggest that the WAT of FSP27-deficient mice have a gene expression profile similar to that of BAT. CONCLUSIONS: FSP27 acts as a molecular determinant that controls gene expression for a diversity of metabolic and signaling pathways and, in particular, the expression of regulatory factors, including CEBPα/β, PRDM16 and components of the cAMP signaling pathway, that control the identity of WAT and BAT. BioMed Central 2010-07-22 /pmc/articles/PMC3091643/ /pubmed/20649970 http://dx.doi.org/10.1186/1471-2164-11-446 Text en Copyright ©2010 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, De
Zhang, Yinxin
Xu, Li
Zhou, Linkang
Wang, Yue
Xue, Bofu
Wen, Zilong
Li, Peng
Sang, Jianli
Regulation of gene expression by FSP27 in white and brown adipose tissue
title Regulation of gene expression by FSP27 in white and brown adipose tissue
title_full Regulation of gene expression by FSP27 in white and brown adipose tissue
title_fullStr Regulation of gene expression by FSP27 in white and brown adipose tissue
title_full_unstemmed Regulation of gene expression by FSP27 in white and brown adipose tissue
title_short Regulation of gene expression by FSP27 in white and brown adipose tissue
title_sort regulation of gene expression by fsp27 in white and brown adipose tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091643/
https://www.ncbi.nlm.nih.gov/pubmed/20649970
http://dx.doi.org/10.1186/1471-2164-11-446
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