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Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile

Tobamoviruses encode a silencing suppressor that binds small RNA (sRNA) duplexes in vitro and supposedly in vivo to counteract antiviral silencing. Here, we used sRNA deep-sequencing combined with transcriptome profiling to determine the global impact of tobamovirus infection on Arabidopsis sRNAs an...

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Autores principales: Hu, Quanan, Hollunder, Jens, Niehl, Annette, Kørner, Camilla Julie, Gereige, Dalya, Windels, David, Arnold, Andreas, Kuiper, Martin, Vazquez, Franck, Pooggin, Mikhail, Heinlein, Manfred
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091872/
https://www.ncbi.nlm.nih.gov/pubmed/21572953
http://dx.doi.org/10.1371/journal.pone.0019549
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author Hu, Quanan
Hollunder, Jens
Niehl, Annette
Kørner, Camilla Julie
Gereige, Dalya
Windels, David
Arnold, Andreas
Kuiper, Martin
Vazquez, Franck
Pooggin, Mikhail
Heinlein, Manfred
author_facet Hu, Quanan
Hollunder, Jens
Niehl, Annette
Kørner, Camilla Julie
Gereige, Dalya
Windels, David
Arnold, Andreas
Kuiper, Martin
Vazquez, Franck
Pooggin, Mikhail
Heinlein, Manfred
author_sort Hu, Quanan
collection PubMed
description Tobamoviruses encode a silencing suppressor that binds small RNA (sRNA) duplexes in vitro and supposedly in vivo to counteract antiviral silencing. Here, we used sRNA deep-sequencing combined with transcriptome profiling to determine the global impact of tobamovirus infection on Arabidopsis sRNAs and their mRNA targets. We found that infection of Arabidopsis plants with Oilseed rape mosaic tobamovirus causes a global size-specific enrichment of miRNAs, ta-siRNAs, and other phased siRNAs. The observed patterns of sRNA enrichment suggest that in addition to a role of the viral silencing suppressor, the stabilization of sRNAs might also occur through association with unknown host effector complexes induced upon infection. Indeed, sRNA enrichment concerns primarily 21-nucleotide RNAs with a 5′-terminal guanine. Interestingly, ORMV infection also leads to accumulation of novel miRNA-like sRNAs from miRNA precursors. Thus, in addition to canonical miRNAs and miRNA*s, miRNA precursors can encode additional sRNAs that may be functional under specific conditions like pathogen infection. Virus-induced sRNA enrichment does not correlate with defects in miRNA-dependent ta-siRNA biogenesis nor with global changes in the levels of mRNA and ta-siRNA targets suggesting that the enriched sRNAs may not be able to significantly contribute to the normal activity of pre-loaded RISC complexes. We conclude that tobamovirus infection induces the stabilization of a specific sRNA pool by yet unknown effector complexes. These complexes may sequester viral and host sRNAs to engage them in yet unknown mechanisms involved in plant:virus interactions.
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spelling pubmed-30918722011-05-13 Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile Hu, Quanan Hollunder, Jens Niehl, Annette Kørner, Camilla Julie Gereige, Dalya Windels, David Arnold, Andreas Kuiper, Martin Vazquez, Franck Pooggin, Mikhail Heinlein, Manfred PLoS One Research Article Tobamoviruses encode a silencing suppressor that binds small RNA (sRNA) duplexes in vitro and supposedly in vivo to counteract antiviral silencing. Here, we used sRNA deep-sequencing combined with transcriptome profiling to determine the global impact of tobamovirus infection on Arabidopsis sRNAs and their mRNA targets. We found that infection of Arabidopsis plants with Oilseed rape mosaic tobamovirus causes a global size-specific enrichment of miRNAs, ta-siRNAs, and other phased siRNAs. The observed patterns of sRNA enrichment suggest that in addition to a role of the viral silencing suppressor, the stabilization of sRNAs might also occur through association with unknown host effector complexes induced upon infection. Indeed, sRNA enrichment concerns primarily 21-nucleotide RNAs with a 5′-terminal guanine. Interestingly, ORMV infection also leads to accumulation of novel miRNA-like sRNAs from miRNA precursors. Thus, in addition to canonical miRNAs and miRNA*s, miRNA precursors can encode additional sRNAs that may be functional under specific conditions like pathogen infection. Virus-induced sRNA enrichment does not correlate with defects in miRNA-dependent ta-siRNA biogenesis nor with global changes in the levels of mRNA and ta-siRNA targets suggesting that the enriched sRNAs may not be able to significantly contribute to the normal activity of pre-loaded RISC complexes. We conclude that tobamovirus infection induces the stabilization of a specific sRNA pool by yet unknown effector complexes. These complexes may sequester viral and host sRNAs to engage them in yet unknown mechanisms involved in plant:virus interactions. Public Library of Science 2011-05-10 /pmc/articles/PMC3091872/ /pubmed/21572953 http://dx.doi.org/10.1371/journal.pone.0019549 Text en Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Quanan
Hollunder, Jens
Niehl, Annette
Kørner, Camilla Julie
Gereige, Dalya
Windels, David
Arnold, Andreas
Kuiper, Martin
Vazquez, Franck
Pooggin, Mikhail
Heinlein, Manfred
Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile
title Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile
title_full Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile
title_fullStr Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile
title_full_unstemmed Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile
title_short Specific Impact of Tobamovirus Infection on the Arabidopsis Small RNA Profile
title_sort specific impact of tobamovirus infection on the arabidopsis small rna profile
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091872/
https://www.ncbi.nlm.nih.gov/pubmed/21572953
http://dx.doi.org/10.1371/journal.pone.0019549
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