Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells

Cathepsin G (CatG) is an endopeptidase that is associated with the early immune response. The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of CatG via a new surface plasmon resonance imaging assay. CGI-I was immobilized on the gold surface of...

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Detalles Bibliográficos
Autores principales: Gorodkiewicz, Ewa, Regulska, Elżbieta, Wojtulewski, Kazimierz
Formato: Texto
Lenguaje:English
Publicado: Springer Vienna 2011
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092066/
https://www.ncbi.nlm.nih.gov/pubmed/21660086
http://dx.doi.org/10.1007/s00604-011-0569-4
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author Gorodkiewicz, Ewa
Regulska, Elżbieta
Wojtulewski, Kazimierz
author_facet Gorodkiewicz, Ewa
Regulska, Elżbieta
Wojtulewski, Kazimierz
author_sort Gorodkiewicz, Ewa
collection PubMed
description Cathepsin G (CatG) is an endopeptidase that is associated with the early immune response. The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of CatG via a new surface plasmon resonance imaging assay. CGI-I was immobilized on the gold surface of an SPR sensor that was first modified with 1-octadecanethiol. A concentration of CGI-I equal to 4.0 μg·mL-1 and a pH of 8.0 were found to give the best results. The dynamic response of the sensor ranges from 0.25 to 1.5 ng·mL-1, and the detection limit is 0.12 ng·mL-1. The sensor was applied to detect CatG in human saliva and white blood cells. [Figure: see text]
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spelling pubmed-30920662011-06-07 Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells Gorodkiewicz, Ewa Regulska, Elżbieta Wojtulewski, Kazimierz Mikrochim Acta Original Paper Cathepsin G (CatG) is an endopeptidase that is associated with the early immune response. The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of CatG via a new surface plasmon resonance imaging assay. CGI-I was immobilized on the gold surface of an SPR sensor that was first modified with 1-octadecanethiol. A concentration of CGI-I equal to 4.0 μg·mL-1 and a pH of 8.0 were found to give the best results. The dynamic response of the sensor ranges from 0.25 to 1.5 ng·mL-1, and the detection limit is 0.12 ng·mL-1. The sensor was applied to detect CatG in human saliva and white blood cells. [Figure: see text] Springer Vienna 2011-03-02 2011 /pmc/articles/PMC3092066/ /pubmed/21660086 http://dx.doi.org/10.1007/s00604-011-0569-4 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Gorodkiewicz, Ewa
Regulska, Elżbieta
Wojtulewski, Kazimierz
Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells
title Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells
title_full Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells
title_fullStr Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells
title_full_unstemmed Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells
title_short Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells
title_sort development of an spr imaging biosensor for determination of cathepsin g in saliva and white blood cells
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092066/
https://www.ncbi.nlm.nih.gov/pubmed/21660086
http://dx.doi.org/10.1007/s00604-011-0569-4
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AT wojtulewskikazimierz developmentofansprimagingbiosensorfordeterminationofcathepsinginsalivaandwhitebloodcells

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