Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET

Anchored ChromPET, a technique to capture and interrogate targeted sequences in the genome, has been developed to identify chromosomal aberrations and define breakpoints. Using this method, we could define the BCR-ABL1 translocation DNA breakpoint to a base-pair resolution in Philadelphia chromosome...

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Detalles Bibliográficos
Autores principales: Shibata, Yoshiyuki, Malhotra, Ankit, Dutta, Anindya
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092121/
https://www.ncbi.nlm.nih.gov/pubmed/20860819
http://dx.doi.org/10.1186/gm191
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author Shibata, Yoshiyuki
Malhotra, Ankit
Dutta, Anindya
author_facet Shibata, Yoshiyuki
Malhotra, Ankit
Dutta, Anindya
author_sort Shibata, Yoshiyuki
collection PubMed
description Anchored ChromPET, a technique to capture and interrogate targeted sequences in the genome, has been developed to identify chromosomal aberrations and define breakpoints. Using this method, we could define the BCR-ABL1 translocation DNA breakpoint to a base-pair resolution in Philadelphia chromosome-positive samples. This DNA-based method is highly sensitive and can detect the fusion junction using samples from which it is hard to obtain RNA or cells where the RNA expression has been silenced.
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spelling pubmed-30921212011-05-12 Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET Shibata, Yoshiyuki Malhotra, Ankit Dutta, Anindya Genome Med Method Anchored ChromPET, a technique to capture and interrogate targeted sequences in the genome, has been developed to identify chromosomal aberrations and define breakpoints. Using this method, we could define the BCR-ABL1 translocation DNA breakpoint to a base-pair resolution in Philadelphia chromosome-positive samples. This DNA-based method is highly sensitive and can detect the fusion junction using samples from which it is hard to obtain RNA or cells where the RNA expression has been silenced. BioMed Central 2010-09-22 /pmc/articles/PMC3092121/ /pubmed/20860819 http://dx.doi.org/10.1186/gm191 Text en Copyright ©2010 Shibata et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Shibata, Yoshiyuki
Malhotra, Ankit
Dutta, Anindya
Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
title Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
title_full Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
title_fullStr Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
title_full_unstemmed Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
title_short Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
title_sort detection of dna fusion junctions for bcr-abl translocations by anchored chrompet
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092121/
https://www.ncbi.nlm.nih.gov/pubmed/20860819
http://dx.doi.org/10.1186/gm191
work_keys_str_mv AT shibatayoshiyuki detectionofdnafusionjunctionsforbcrabltranslocationsbyanchoredchrompet
AT malhotraankit detectionofdnafusionjunctionsforbcrabltranslocationsbyanchoredchrompet
AT duttaanindya detectionofdnafusionjunctionsforbcrabltranslocationsbyanchoredchrompet

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