Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus

Dehydroquinate synthase (DHQS) catalyses the second step of the shikimate pathway to aromatic compounds. DHQS from the archaeal hyperthermophile Pyrococcus furiosus was insoluble when expressed in Escherichia coli but was partially solubilised when KCl was included in the cell lysis buffer. A purifi...

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Detalles Bibliográficos
Autores principales: Negron, Leonardo, Patchett, Mark L., Parker, Emily J.
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092513/
https://www.ncbi.nlm.nih.gov/pubmed/21603259
http://dx.doi.org/10.4061/2011/134893

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092513/
https://www.ncbi.nlm.nih.gov/pubmed/21603259
http://dx.doi.org/10.4061/2011/134893

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