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Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice

Asiatic acid (AA), a pentacyclic triterpene compound in the medicinal plant Centella asiatica, was evaluated for antinociceptive and anti-inflammatory effects. Treatment of male ICR mice with AA significantly inhibited the numbers of acetic acid-induced writhing responses and the formalin-induced pa...

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Autores principales: Huang, Shyh-Shyun, Chiu, Chuan-Sung, Chen, Hsien-Jung, Hou, Wen-Chi, Sheu, Ming-Jyh, Lin, Ying-Chih, Shie, Pei-Hsin, Huang, Guan-Jhong
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092715/
https://www.ncbi.nlm.nih.gov/pubmed/21584194
http://dx.doi.org/10.1155/2011/895857
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author Huang, Shyh-Shyun
Chiu, Chuan-Sung
Chen, Hsien-Jung
Hou, Wen-Chi
Sheu, Ming-Jyh
Lin, Ying-Chih
Shie, Pei-Hsin
Huang, Guan-Jhong
author_facet Huang, Shyh-Shyun
Chiu, Chuan-Sung
Chen, Hsien-Jung
Hou, Wen-Chi
Sheu, Ming-Jyh
Lin, Ying-Chih
Shie, Pei-Hsin
Huang, Guan-Jhong
author_sort Huang, Shyh-Shyun
collection PubMed
description Asiatic acid (AA), a pentacyclic triterpene compound in the medicinal plant Centella asiatica, was evaluated for antinociceptive and anti-inflammatory effects. Treatment of male ICR mice with AA significantly inhibited the numbers of acetic acid-induced writhing responses and the formalin-induced pain in the late phase. In the anti-inflammatory test, AA decreased the paw edema at the 4th and 5th h after λ-carrageenan (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. AA decreased the nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) levels on serum level at the 5th h after Carr injection. Western blotting revealed that AA decreased Carr-induced inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and nuclear factor-κB (NF-κB) expressions at the 5th h in the edema paw. An intraperitoneal (i.p.) injection treatment with AA also diminished neutrophil infiltration into sites of inflammation as did indomethacin (Indo). The anti-inflammatory mechanisms of AA might be related to the decrease in the level of MDA, iNOS, COX-2, and NF-κB in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver.
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spelling pubmed-30927152011-05-16 Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice Huang, Shyh-Shyun Chiu, Chuan-Sung Chen, Hsien-Jung Hou, Wen-Chi Sheu, Ming-Jyh Lin, Ying-Chih Shie, Pei-Hsin Huang, Guan-Jhong Evid Based Complement Alternat Med Research Article Asiatic acid (AA), a pentacyclic triterpene compound in the medicinal plant Centella asiatica, was evaluated for antinociceptive and anti-inflammatory effects. Treatment of male ICR mice with AA significantly inhibited the numbers of acetic acid-induced writhing responses and the formalin-induced pain in the late phase. In the anti-inflammatory test, AA decreased the paw edema at the 4th and 5th h after λ-carrageenan (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. AA decreased the nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) levels on serum level at the 5th h after Carr injection. Western blotting revealed that AA decreased Carr-induced inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and nuclear factor-κB (NF-κB) expressions at the 5th h in the edema paw. An intraperitoneal (i.p.) injection treatment with AA also diminished neutrophil infiltration into sites of inflammation as did indomethacin (Indo). The anti-inflammatory mechanisms of AA might be related to the decrease in the level of MDA, iNOS, COX-2, and NF-κB in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver. Hindawi Publishing Corporation 2011 2011-04-20 /pmc/articles/PMC3092715/ /pubmed/21584194 http://dx.doi.org/10.1155/2011/895857 Text en Copyright © 2011 Shyh-Shyun Huang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Shyh-Shyun
Chiu, Chuan-Sung
Chen, Hsien-Jung
Hou, Wen-Chi
Sheu, Ming-Jyh
Lin, Ying-Chih
Shie, Pei-Hsin
Huang, Guan-Jhong
Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice
title Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice
title_full Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice
title_fullStr Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice
title_full_unstemmed Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice
title_short Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice
title_sort antinociceptive activities and the mechanisms of anti-inflammation of asiatic acid in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092715/
https://www.ncbi.nlm.nih.gov/pubmed/21584194
http://dx.doi.org/10.1155/2011/895857
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