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Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy

Prions are largely contained within the nervous and lymphoid tissue of transmissible spongiform encephalopathy (TSE) infected animals. However, following advances in diagnostic sensitivity, PrP(Sc), a marker for prion disease, can now be located in a wide range of viscera and body fluids including m...

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Autores principales: Everest, Sally J., Ramsay, Andrew M., Chaplin, Melanie J., Everitt, Sharon, Stack, Michael J., Neale, Michael H., Jeffrey, Martin, Moore, S. Jo, Bellworthy, Susan J., Terry, Linda A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093390/
https://www.ncbi.nlm.nih.gov/pubmed/21589864
http://dx.doi.org/10.1371/journal.pone.0019737
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author Everest, Sally J.
Ramsay, Andrew M.
Chaplin, Melanie J.
Everitt, Sharon
Stack, Michael J.
Neale, Michael H.
Jeffrey, Martin
Moore, S. Jo
Bellworthy, Susan J.
Terry, Linda A.
author_facet Everest, Sally J.
Ramsay, Andrew M.
Chaplin, Melanie J.
Everitt, Sharon
Stack, Michael J.
Neale, Michael H.
Jeffrey, Martin
Moore, S. Jo
Bellworthy, Susan J.
Terry, Linda A.
author_sort Everest, Sally J.
collection PubMed
description Prions are largely contained within the nervous and lymphoid tissue of transmissible spongiform encephalopathy (TSE) infected animals. However, following advances in diagnostic sensitivity, PrP(Sc), a marker for prion disease, can now be located in a wide range of viscera and body fluids including muscle, saliva, blood, urine and milk, raising concerns that exposure to these materials could contribute to the spread of disease in humans and animals. Previously we demonstrated low levels of infectivity in the liver of sheep experimentally challenged with bovine spongiform encephalopathy. In this study we show that PrP(Sc) accumulated in the liver of 89% of sheep naturally infected with scrapie and 100% of sheep challenged with BSE, at both clinical and preclinical stages of the disease. PrP(Sc) was demonstrated in the absence of obvious inflammatory foci and was restricted to isolated resident cells, most likely Kupffer cells.
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spelling pubmed-30933902011-05-17 Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy Everest, Sally J. Ramsay, Andrew M. Chaplin, Melanie J. Everitt, Sharon Stack, Michael J. Neale, Michael H. Jeffrey, Martin Moore, S. Jo Bellworthy, Susan J. Terry, Linda A. PLoS One Research Article Prions are largely contained within the nervous and lymphoid tissue of transmissible spongiform encephalopathy (TSE) infected animals. However, following advances in diagnostic sensitivity, PrP(Sc), a marker for prion disease, can now be located in a wide range of viscera and body fluids including muscle, saliva, blood, urine and milk, raising concerns that exposure to these materials could contribute to the spread of disease in humans and animals. Previously we demonstrated low levels of infectivity in the liver of sheep experimentally challenged with bovine spongiform encephalopathy. In this study we show that PrP(Sc) accumulated in the liver of 89% of sheep naturally infected with scrapie and 100% of sheep challenged with BSE, at both clinical and preclinical stages of the disease. PrP(Sc) was demonstrated in the absence of obvious inflammatory foci and was restricted to isolated resident cells, most likely Kupffer cells. Public Library of Science 2011-05-12 /pmc/articles/PMC3093390/ /pubmed/21589864 http://dx.doi.org/10.1371/journal.pone.0019737 Text en Everest et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Everest, Sally J.
Ramsay, Andrew M.
Chaplin, Melanie J.
Everitt, Sharon
Stack, Michael J.
Neale, Michael H.
Jeffrey, Martin
Moore, S. Jo
Bellworthy, Susan J.
Terry, Linda A.
Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy
title Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy
title_full Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy
title_fullStr Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy
title_full_unstemmed Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy
title_short Detection and Localisation of PrP(Sc) in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy
title_sort detection and localisation of prp(sc) in the liver of sheep infected with scrapie and bovine spongiform encephalopathy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093390/
https://www.ncbi.nlm.nih.gov/pubmed/21589864
http://dx.doi.org/10.1371/journal.pone.0019737
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