Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

BACKGROUND: Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled receptors (GPCRs) for structural studies. Rec...

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Detalles Bibliográficos
Autores principales: Asada, Hidetsugu, Uemura, Tomoko, Yurugi-Kobayashi, Takami, Shiroishi, Mitsunori, Shimamura, Tatsuro, Tsujimoto, Hirokazu, Ito, Keisuke, Sugawara, Taishi, Nakane, Takanori, Nomura, Norimichi, Murata, Takeshi, Haga, Tatsuya, Iwata, So, Kobayashi, Takuya
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094209/
https://www.ncbi.nlm.nih.gov/pubmed/21513509
http://dx.doi.org/10.1186/1475-2859-10-24

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