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Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers

BACKGROUND: Occupational exposure to chromium compounds may result in adverse health effects. This study aims to investigate whether low-level hexavalent chromium (Cr(VI)) exposure can cause DNA damage in electroplating workers. METHODS: 157 electroplating workers and 93 control subjects with no his...

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Autores principales: Zhang, Xu-Hui, Zhang, Xuan, Wang, Xu-Chu, Jin, Li-Fen, Yang, Zhang-Ping, Jiang, Cai-Xia, Chen, Qing, Ren, Xiao-Bin, Cao, Jian-Zhong, Wang, Qiang, Zhu, Yi-Min
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094242/
https://www.ncbi.nlm.nih.gov/pubmed/21481275
http://dx.doi.org/10.1186/1471-2458-11-224
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author Zhang, Xu-Hui
Zhang, Xuan
Wang, Xu-Chu
Jin, Li-Fen
Yang, Zhang-Ping
Jiang, Cai-Xia
Chen, Qing
Ren, Xiao-Bin
Cao, Jian-Zhong
Wang, Qiang
Zhu, Yi-Min
author_facet Zhang, Xu-Hui
Zhang, Xuan
Wang, Xu-Chu
Jin, Li-Fen
Yang, Zhang-Ping
Jiang, Cai-Xia
Chen, Qing
Ren, Xiao-Bin
Cao, Jian-Zhong
Wang, Qiang
Zhu, Yi-Min
author_sort Zhang, Xu-Hui
collection PubMed
description BACKGROUND: Occupational exposure to chromium compounds may result in adverse health effects. This study aims to investigate whether low-level hexavalent chromium (Cr(VI)) exposure can cause DNA damage in electroplating workers. METHODS: 157 electroplating workers and 93 control subjects with no history of occupational exposure to chromium were recruited in Hangzhou, China. Chromium levels in erythrocytes were determined by graphite furnace atomic absorption spectrophotometer. DNA damage in peripheral lymphocytes was evaluated with the alkaline comet assay by three parameters: Olive tail moment, tail length and percent of DNA in the comet tail (tail DNA%). Urinary 8-OHdG levels were measured by ELISA. RESULTS: Chromium concentration in erythrocytes was about two times higher in electroplating workers (median: 4.41 μg/L) than that in control subjects (1.54 μg/L, P < 0.001). The medians (range) of Olive tail moment, tail length and tail DNA% in exposed workers were 1.13 (0.14-6.77), 11.17 (3.46-52.19) and 3.69 (0.65-16.20), and were significantly higher than those in control subjects (0.14 (0.01-0.39), 3.26 (3.00-4.00) and 0.69 (0.04-2.74), P < 0.001). Urinary 8-OHdG concentration was 13.65 (3.08-66.30) μg/g creatinine in exposed workers and 8.31 (2.94-30.83) μg/g creatinine in control subjects (P < 0.001). The differences of urinary 8-OHdG levels, Olive tail moment, tail length and tail DNA% between these two groups remained significant (P < 0.001) even after stratification by potential confounding factors such as age, gender, and smoking status. Chromium exposure was found to be positively associated with chromium levels in erythrocytes, urinary 8-OHdG levels, Olive tail moment, tail length and tail DNA%. Positive dose-response associations were also found between chromium levels in erythrocytes and Olive tail moment, tail length and tail DNA%. CONCLUSION: The findings in this study indicated that there was detectable chromium exposure in electroplating workers. Low-level occupational chromium exposure induced DNA damage.
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spelling pubmed-30942422011-05-14 Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers Zhang, Xu-Hui Zhang, Xuan Wang, Xu-Chu Jin, Li-Fen Yang, Zhang-Ping Jiang, Cai-Xia Chen, Qing Ren, Xiao-Bin Cao, Jian-Zhong Wang, Qiang Zhu, Yi-Min BMC Public Health Research Article BACKGROUND: Occupational exposure to chromium compounds may result in adverse health effects. This study aims to investigate whether low-level hexavalent chromium (Cr(VI)) exposure can cause DNA damage in electroplating workers. METHODS: 157 electroplating workers and 93 control subjects with no history of occupational exposure to chromium were recruited in Hangzhou, China. Chromium levels in erythrocytes were determined by graphite furnace atomic absorption spectrophotometer. DNA damage in peripheral lymphocytes was evaluated with the alkaline comet assay by three parameters: Olive tail moment, tail length and percent of DNA in the comet tail (tail DNA%). Urinary 8-OHdG levels were measured by ELISA. RESULTS: Chromium concentration in erythrocytes was about two times higher in electroplating workers (median: 4.41 μg/L) than that in control subjects (1.54 μg/L, P < 0.001). The medians (range) of Olive tail moment, tail length and tail DNA% in exposed workers were 1.13 (0.14-6.77), 11.17 (3.46-52.19) and 3.69 (0.65-16.20), and were significantly higher than those in control subjects (0.14 (0.01-0.39), 3.26 (3.00-4.00) and 0.69 (0.04-2.74), P < 0.001). Urinary 8-OHdG concentration was 13.65 (3.08-66.30) μg/g creatinine in exposed workers and 8.31 (2.94-30.83) μg/g creatinine in control subjects (P < 0.001). The differences of urinary 8-OHdG levels, Olive tail moment, tail length and tail DNA% between these two groups remained significant (P < 0.001) even after stratification by potential confounding factors such as age, gender, and smoking status. Chromium exposure was found to be positively associated with chromium levels in erythrocytes, urinary 8-OHdG levels, Olive tail moment, tail length and tail DNA%. Positive dose-response associations were also found between chromium levels in erythrocytes and Olive tail moment, tail length and tail DNA%. CONCLUSION: The findings in this study indicated that there was detectable chromium exposure in electroplating workers. Low-level occupational chromium exposure induced DNA damage. BioMed Central 2011-04-12 /pmc/articles/PMC3094242/ /pubmed/21481275 http://dx.doi.org/10.1186/1471-2458-11-224 Text en Copyright ©2011 Zhang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Xu-Hui
Zhang, Xuan
Wang, Xu-Chu
Jin, Li-Fen
Yang, Zhang-Ping
Jiang, Cai-Xia
Chen, Qing
Ren, Xiao-Bin
Cao, Jian-Zhong
Wang, Qiang
Zhu, Yi-Min
Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers
title Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers
title_full Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers
title_fullStr Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers
title_full_unstemmed Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers
title_short Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers
title_sort chronic occupational exposure to hexavalent chromium causes dna damage in electroplating workers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094242/
https://www.ncbi.nlm.nih.gov/pubmed/21481275
http://dx.doi.org/10.1186/1471-2458-11-224
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