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Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3

BACKGROUND: Many of researchers have focused on the emerging pathogen, Norovirus, since its first identification as the causing agent of nonbacterial acute gastroenteritis in humans. One of the virulence factors of norovirus, the great genetic diversity attributed to point mutations and recombinatio...

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Autores principales: Park, Jeong-Woong, Lee, Sung-Geun, Lee, Young-Min, Jheong, Weon-Hwa, Ryu, Sangryeol, Paik, Soon-Young
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094297/
https://www.ncbi.nlm.nih.gov/pubmed/21489302
http://dx.doi.org/10.1186/1743-422X-8-167
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author Park, Jeong-Woong
Lee, Sung-Geun
Lee, Young-Min
Jheong, Weon-Hwa
Ryu, Sangryeol
Paik, Soon-Young
author_facet Park, Jeong-Woong
Lee, Sung-Geun
Lee, Young-Min
Jheong, Weon-Hwa
Ryu, Sangryeol
Paik, Soon-Young
author_sort Park, Jeong-Woong
collection PubMed
description BACKGROUND: Many of researchers have focused on the emerging pathogen, Norovirus, since its first identification as the causing agent of nonbacterial acute gastroenteritis in humans. One of the virulence factors of norovirus, the great genetic diversity attributed to point mutations and recombinations, has brought forth the result of significant changes in the circulating norovirus genotype patterns. FINDINGS: In recognition of the necessity for tracking and monitoring of genetic diversity, a norovirus variant among the most prevalent genotype GII-4, Norovirus Hu/GII-4/CUK-3/2008/KR (CUK-3), was isolated from stool samples and analyzed on the level of whole genome sequence. Whole genome sequence analysis revealed three ORF composites of the whole genome, ORF1 (5100 bp), ORF2 (1623 bp), and ORF3 (807 bp). Each genetic relationship of CUK-3 variant analysis located the ORF1 (5,100 bp) in Cluster I, ORF2 (1623 bp) in Cluster I (2006b), ORF3 (807 bp) in Cluster I, and the whole genome sequence (about 5.1 kb) in Cluster I in the phylogenetic tree. And the phylogenetic analyses showed the same location of CUK-3 strain with the GII-4/2006b cluster in the phylogenetic tree. CONCLUSIONS: In This study, a first concerning the full-length sequence of a NoV variant in South Korea is meaningful in that it can be used not only as a full-length NoV variant sequence standard for future comparison studies, but also as useful material for the public health field by enabling the diagnosis, vaccine development, and prediction of new emerging variants.
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spelling pubmed-30942972011-05-14 Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3 Park, Jeong-Woong Lee, Sung-Geun Lee, Young-Min Jheong, Weon-Hwa Ryu, Sangryeol Paik, Soon-Young Virol J Short Report BACKGROUND: Many of researchers have focused on the emerging pathogen, Norovirus, since its first identification as the causing agent of nonbacterial acute gastroenteritis in humans. One of the virulence factors of norovirus, the great genetic diversity attributed to point mutations and recombinations, has brought forth the result of significant changes in the circulating norovirus genotype patterns. FINDINGS: In recognition of the necessity for tracking and monitoring of genetic diversity, a norovirus variant among the most prevalent genotype GII-4, Norovirus Hu/GII-4/CUK-3/2008/KR (CUK-3), was isolated from stool samples and analyzed on the level of whole genome sequence. Whole genome sequence analysis revealed three ORF composites of the whole genome, ORF1 (5100 bp), ORF2 (1623 bp), and ORF3 (807 bp). Each genetic relationship of CUK-3 variant analysis located the ORF1 (5,100 bp) in Cluster I, ORF2 (1623 bp) in Cluster I (2006b), ORF3 (807 bp) in Cluster I, and the whole genome sequence (about 5.1 kb) in Cluster I in the phylogenetic tree. And the phylogenetic analyses showed the same location of CUK-3 strain with the GII-4/2006b cluster in the phylogenetic tree. CONCLUSIONS: In This study, a first concerning the full-length sequence of a NoV variant in South Korea is meaningful in that it can be used not only as a full-length NoV variant sequence standard for future comparison studies, but also as useful material for the public health field by enabling the diagnosis, vaccine development, and prediction of new emerging variants. BioMed Central 2011-04-14 /pmc/articles/PMC3094297/ /pubmed/21489302 http://dx.doi.org/10.1186/1743-422X-8-167 Text en Copyright ©2011 Park et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Park, Jeong-Woong
Lee, Sung-Geun
Lee, Young-Min
Jheong, Weon-Hwa
Ryu, Sangryeol
Paik, Soon-Young
Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3
title Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3
title_full Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3
title_fullStr Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3
title_full_unstemmed Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3
title_short Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3
title_sort full sequence analysis and characterization of the south korean norovirus gii-4 variant cuk-3
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094297/
https://www.ncbi.nlm.nih.gov/pubmed/21489302
http://dx.doi.org/10.1186/1743-422X-8-167
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