The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens

Urine is the CDC-recommended specimen for STI testing. It was unknown if the bacterial communities (microbiomes) in urine reflected those in the distal male urethra. We compared microbiomes of 32 paired urine and urethral swab specimens obtained from adult men attending an STD clinic, by 16S rRNA PC...

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Detalles Bibliográficos
Autores principales: Dong, Qunfeng, Nelson, David E., Toh, Evelyn, Diao, Lixia, Gao, Xiang, Fortenberry, J. Dennis, Van Der Pol, Barbara
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094389/
https://www.ncbi.nlm.nih.gov/pubmed/21603636
http://dx.doi.org/10.1371/journal.pone.0019709
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author Dong, Qunfeng
Nelson, David E.
Toh, Evelyn
Diao, Lixia
Gao, Xiang
Fortenberry, J. Dennis
Van Der Pol, Barbara
author_facet Dong, Qunfeng
Nelson, David E.
Toh, Evelyn
Diao, Lixia
Gao, Xiang
Fortenberry, J. Dennis
Van Der Pol, Barbara
author_sort Dong, Qunfeng
collection PubMed
description Urine is the CDC-recommended specimen for STI testing. It was unknown if the bacterial communities (microbiomes) in urine reflected those in the distal male urethra. We compared microbiomes of 32 paired urine and urethral swab specimens obtained from adult men attending an STD clinic, by 16S rRNA PCR and deep pyrosequencing. Microbiomes of urine and swabs were remarkably similar, regardless of STI status of the subjects. Thus, urine can be used to characterize urethral microbiomes when swabs are undesirable, such as in population-based studies of the urethral microbiome or where multiple sampling of participants is required.
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spelling pubmed-30943892011-05-19 The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens Dong, Qunfeng Nelson, David E. Toh, Evelyn Diao, Lixia Gao, Xiang Fortenberry, J. Dennis Van Der Pol, Barbara PLoS One Research Article Urine is the CDC-recommended specimen for STI testing. It was unknown if the bacterial communities (microbiomes) in urine reflected those in the distal male urethra. We compared microbiomes of 32 paired urine and urethral swab specimens obtained from adult men attending an STD clinic, by 16S rRNA PCR and deep pyrosequencing. Microbiomes of urine and swabs were remarkably similar, regardless of STI status of the subjects. Thus, urine can be used to characterize urethral microbiomes when swabs are undesirable, such as in population-based studies of the urethral microbiome or where multiple sampling of participants is required. Public Library of Science 2011-05-13 /pmc/articles/PMC3094389/ /pubmed/21603636 http://dx.doi.org/10.1371/journal.pone.0019709 Text en Dong et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dong, Qunfeng
Nelson, David E.
Toh, Evelyn
Diao, Lixia
Gao, Xiang
Fortenberry, J. Dennis
Van Der Pol, Barbara
The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens
title The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens
title_full The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens
title_fullStr The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens
title_full_unstemmed The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens
title_short The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens
title_sort microbial communities in male first catch urine are highly similar to those in paired urethral swab specimens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094389/
https://www.ncbi.nlm.nih.gov/pubmed/21603636
http://dx.doi.org/10.1371/journal.pone.0019709
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