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An in silico analysis of the mitochondrial protein import apparatus of plants

BACKGROUND: An in silico analysis of the mitochondrial protein import apparatus from a variety of species; including Chlamydomonas reinhardtii, Chlorella variabilis, Ectocarpus siliculosus, Cyanidioschyzon merolae, Physcomitrella patens, Selaginella moellendorffii, Picea glauca, Oryza sativa and Ara...

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Autores principales: Carrie, Chris, Murcha, Monika W, Whelan, James
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095331/
https://www.ncbi.nlm.nih.gov/pubmed/21078193
http://dx.doi.org/10.1186/1471-2229-10-249
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author Carrie, Chris
Murcha, Monika W
Whelan, James
author_facet Carrie, Chris
Murcha, Monika W
Whelan, James
author_sort Carrie, Chris
collection PubMed
description BACKGROUND: An in silico analysis of the mitochondrial protein import apparatus from a variety of species; including Chlamydomonas reinhardtii, Chlorella variabilis, Ectocarpus siliculosus, Cyanidioschyzon merolae, Physcomitrella patens, Selaginella moellendorffii, Picea glauca, Oryza sativa and Arabidopsis thaliana was undertaken to determine if components differed within and between plant and non-plant species. RESULTS: The channel forming subunits of the outer membrane components Tom40 and Sam50 are conserved between plant groups and other eukaryotes. In contrast, the receptor component(s) in green plants, particularly Tom20, (C. reinhardtii, C. variabilis, P. patens, S. moellendorffii, P. glauca, O. sativa and A. thaliana) are specific to this lineage. Red algae contain a Tom22 receptor that is orthologous to yeast Tom22. Furthermore, plant mitochondrial receptors display differences between various plant lineages. These are evidenced by distinctive motifs in all plant Metaxins, which are absent in red algae, and the presence of the outer membrane receptor OM64 in Angiosperms (rice and Arabidopsis), but not in lycophytes (S. moellendorffii) and gymnosperms (P. glauca). Furthermore, although the intermembrane space receptor Mia40 is conserved across a wide phylogenetic range, its function differs between lineages. In all plant lineages, Tim17 contains a C-terminal extension, which may act as a receptor component for the import of nucleic acids into plant mitochondria. CONCLUSIONS: It is proposed that the observed functional divergences are due to the selective pressure to sort proteins between mitochondria and chloroplasts, resulting in differences in protein receptor components between plant groups and other organisms. Additionally, diversity of receptor components is observed within the plant kingdom. Even when receptor components are orthologous across plant and non-plant species, it appears that the functions of these have expanded or diverged in a lineage specific manner.
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spelling pubmed-30953312011-05-17 An in silico analysis of the mitochondrial protein import apparatus of plants Carrie, Chris Murcha, Monika W Whelan, James BMC Plant Biol Research Article BACKGROUND: An in silico analysis of the mitochondrial protein import apparatus from a variety of species; including Chlamydomonas reinhardtii, Chlorella variabilis, Ectocarpus siliculosus, Cyanidioschyzon merolae, Physcomitrella patens, Selaginella moellendorffii, Picea glauca, Oryza sativa and Arabidopsis thaliana was undertaken to determine if components differed within and between plant and non-plant species. RESULTS: The channel forming subunits of the outer membrane components Tom40 and Sam50 are conserved between plant groups and other eukaryotes. In contrast, the receptor component(s) in green plants, particularly Tom20, (C. reinhardtii, C. variabilis, P. patens, S. moellendorffii, P. glauca, O. sativa and A. thaliana) are specific to this lineage. Red algae contain a Tom22 receptor that is orthologous to yeast Tom22. Furthermore, plant mitochondrial receptors display differences between various plant lineages. These are evidenced by distinctive motifs in all plant Metaxins, which are absent in red algae, and the presence of the outer membrane receptor OM64 in Angiosperms (rice and Arabidopsis), but not in lycophytes (S. moellendorffii) and gymnosperms (P. glauca). Furthermore, although the intermembrane space receptor Mia40 is conserved across a wide phylogenetic range, its function differs between lineages. In all plant lineages, Tim17 contains a C-terminal extension, which may act as a receptor component for the import of nucleic acids into plant mitochondria. CONCLUSIONS: It is proposed that the observed functional divergences are due to the selective pressure to sort proteins between mitochondria and chloroplasts, resulting in differences in protein receptor components between plant groups and other organisms. Additionally, diversity of receptor components is observed within the plant kingdom. Even when receptor components are orthologous across plant and non-plant species, it appears that the functions of these have expanded or diverged in a lineage specific manner. BioMed Central 2010-11-16 /pmc/articles/PMC3095331/ /pubmed/21078193 http://dx.doi.org/10.1186/1471-2229-10-249 Text en Copyright ©2010 Carrie et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Carrie, Chris
Murcha, Monika W
Whelan, James
An in silico analysis of the mitochondrial protein import apparatus of plants
title An in silico analysis of the mitochondrial protein import apparatus of plants
title_full An in silico analysis of the mitochondrial protein import apparatus of plants
title_fullStr An in silico analysis of the mitochondrial protein import apparatus of plants
title_full_unstemmed An in silico analysis of the mitochondrial protein import apparatus of plants
title_short An in silico analysis of the mitochondrial protein import apparatus of plants
title_sort in silico analysis of the mitochondrial protein import apparatus of plants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095331/
https://www.ncbi.nlm.nih.gov/pubmed/21078193
http://dx.doi.org/10.1186/1471-2229-10-249
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