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The role of actin isoforms in somatic embryogenesis in Norway spruce

BACKGROUND: Somatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Nor...

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Autores principales: Schwarzerová, Kateřina, Vondráková, Zuzana, Fischer, Lukáš, Boříková, Petra, Bellinvia, Erica, Eliášová, Kateřina, Havelková, Lenka, Fišerová, Jindřiška, Vágner, Martin, Opatrný, Zdeněk
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095357/
https://www.ncbi.nlm.nih.gov/pubmed/20478025
http://dx.doi.org/10.1186/1471-2229-10-89
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author Schwarzerová, Kateřina
Vondráková, Zuzana
Fischer, Lukáš
Boříková, Petra
Bellinvia, Erica
Eliášová, Kateřina
Havelková, Lenka
Fišerová, Jindřiška
Vágner, Martin
Opatrný, Zdeněk
author_facet Schwarzerová, Kateřina
Vondráková, Zuzana
Fischer, Lukáš
Boříková, Petra
Bellinvia, Erica
Eliášová, Kateřina
Havelková, Lenka
Fišerová, Jindřiška
Vágner, Martin
Opatrný, Zdeněk
author_sort Schwarzerová, Kateřina
collection PubMed
description BACKGROUND: Somatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Norway spruce line AFO 541 by means of anti-actin drugs. RESULTS: Application of low doses (50-100 nM) of latrunculin B (Lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of more advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. In searching for the cause of the differential actin sensitivity of the two cell types, we analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was greatly enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B. CONCLUSIONS: We show that manipulating actin in specific cell types in somatic embryos using Lat B treatment accelerated and even synchronized the development of somatic embryos and may be of practical use in biotechnology.
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spelling pubmed-30953572011-05-17 The role of actin isoforms in somatic embryogenesis in Norway spruce Schwarzerová, Kateřina Vondráková, Zuzana Fischer, Lukáš Boříková, Petra Bellinvia, Erica Eliášová, Kateřina Havelková, Lenka Fišerová, Jindřiška Vágner, Martin Opatrný, Zdeněk BMC Plant Biol Research Article BACKGROUND: Somatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Norway spruce line AFO 541 by means of anti-actin drugs. RESULTS: Application of low doses (50-100 nM) of latrunculin B (Lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of more advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. In searching for the cause of the differential actin sensitivity of the two cell types, we analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was greatly enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B. CONCLUSIONS: We show that manipulating actin in specific cell types in somatic embryos using Lat B treatment accelerated and even synchronized the development of somatic embryos and may be of practical use in biotechnology. BioMed Central 2010-05-17 /pmc/articles/PMC3095357/ /pubmed/20478025 http://dx.doi.org/10.1186/1471-2229-10-89 Text en Copyright ©2010 Schwarzerová et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Schwarzerová, Kateřina
Vondráková, Zuzana
Fischer, Lukáš
Boříková, Petra
Bellinvia, Erica
Eliášová, Kateřina
Havelková, Lenka
Fišerová, Jindřiška
Vágner, Martin
Opatrný, Zdeněk
The role of actin isoforms in somatic embryogenesis in Norway spruce
title The role of actin isoforms in somatic embryogenesis in Norway spruce
title_full The role of actin isoforms in somatic embryogenesis in Norway spruce
title_fullStr The role of actin isoforms in somatic embryogenesis in Norway spruce
title_full_unstemmed The role of actin isoforms in somatic embryogenesis in Norway spruce
title_short The role of actin isoforms in somatic embryogenesis in Norway spruce
title_sort role of actin isoforms in somatic embryogenesis in norway spruce
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095357/
https://www.ncbi.nlm.nih.gov/pubmed/20478025
http://dx.doi.org/10.1186/1471-2229-10-89
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