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Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid

The present studies were focused on the formation and characterization of sterically stabilized archaeosomes made from a synthetic PEGylated archaeal lipid. In a first step, a synthetic archaeal tetraether bipolar lipid was functionalized with a poly(ethylene glycol), PEG, and (PEG(45)-Tetraether) w...

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Detalles Bibliográficos
Autores principales: Barbeau, Julie, Cammas-Marion, Sandrine, Auvray, Pierrick, Benvegnu, Thierry
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095417/
https://www.ncbi.nlm.nih.gov/pubmed/21603209
http://dx.doi.org/10.1155/2011/396068
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author Barbeau, Julie
Cammas-Marion, Sandrine
Auvray, Pierrick
Benvegnu, Thierry
author_facet Barbeau, Julie
Cammas-Marion, Sandrine
Auvray, Pierrick
Benvegnu, Thierry
author_sort Barbeau, Julie
collection PubMed
description The present studies were focused on the formation and characterization of sterically stabilized archaeosomes made from a synthetic PEGylated archaeal lipid. In a first step, a synthetic archaeal tetraether bipolar lipid was functionalized with a poly(ethylene glycol), PEG, and (PEG(45)-Tetraether) with the aim of coating the archaeosome surface with a sterically stabilizing hydrophilic polymer. In a second step, Egg-PC/PEG(45)-Tetraether (90/10 wt%) archaeosomes were prepared, and their physicochemical characteristics were determined by dynamic light scattering (size, polydispersity), cryo-TEM (morphology), and by high-performance thin layer chromatography (lipid composition), in comparison with standard Egg-PC/PEG(45)-DSPE formulations. Further, a fluorescent dye, the carboxyfluorescein, was encapsulated into the prepared archaeosomes in order to evaluate the potential of such nanostructures as drug carriers. Release studies have shown that the stability of Egg-PC/PEG(45)-Tetraether-based archaeosomes is significantly higher at 37°C than the one of Egg-PC/PEG(45)-DSPE-based liposomes, as evidenced by the slower release of the dye encapsulated into PEGylated archaeosomes. This enhanced stability could be related to the membrane spanning properties of the archaeal bipolar lipid as already described with natural or synthetic tetraether lipids.
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spelling pubmed-30954172011-05-20 Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid Barbeau, Julie Cammas-Marion, Sandrine Auvray, Pierrick Benvegnu, Thierry J Drug Deliv Research Article The present studies were focused on the formation and characterization of sterically stabilized archaeosomes made from a synthetic PEGylated archaeal lipid. In a first step, a synthetic archaeal tetraether bipolar lipid was functionalized with a poly(ethylene glycol), PEG, and (PEG(45)-Tetraether) with the aim of coating the archaeosome surface with a sterically stabilizing hydrophilic polymer. In a second step, Egg-PC/PEG(45)-Tetraether (90/10 wt%) archaeosomes were prepared, and their physicochemical characteristics were determined by dynamic light scattering (size, polydispersity), cryo-TEM (morphology), and by high-performance thin layer chromatography (lipid composition), in comparison with standard Egg-PC/PEG(45)-DSPE formulations. Further, a fluorescent dye, the carboxyfluorescein, was encapsulated into the prepared archaeosomes in order to evaluate the potential of such nanostructures as drug carriers. Release studies have shown that the stability of Egg-PC/PEG(45)-Tetraether-based archaeosomes is significantly higher at 37°C than the one of Egg-PC/PEG(45)-DSPE-based liposomes, as evidenced by the slower release of the dye encapsulated into PEGylated archaeosomes. This enhanced stability could be related to the membrane spanning properties of the archaeal bipolar lipid as already described with natural or synthetic tetraether lipids. Hindawi Publishing Corporation 2011 2011-03-21 /pmc/articles/PMC3095417/ /pubmed/21603209 http://dx.doi.org/10.1155/2011/396068 Text en Copyright © 2011 Julie Barbeau et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Barbeau, Julie
Cammas-Marion, Sandrine
Auvray, Pierrick
Benvegnu, Thierry
Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid
title Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid
title_full Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid
title_fullStr Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid
title_full_unstemmed Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid
title_short Preparation and Characterization of Stealth Archaeosomes Based on a Synthetic PEGylated Archaeal Tetraether Lipid
title_sort preparation and characterization of stealth archaeosomes based on a synthetic pegylated archaeal tetraether lipid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095417/
https://www.ncbi.nlm.nih.gov/pubmed/21603209
http://dx.doi.org/10.1155/2011/396068
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