Cargando…
Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells
Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096473/ https://www.ncbi.nlm.nih.gov/pubmed/20007262 http://dx.doi.org/10.1093/ecam/nep188 |
_version_ | 1782203720379924480 |
---|---|
author | Kataria, Hardeep Shah, Navjot Kaul, Sunil C. Wadhwa, Renu Kaur, Gurcharan |
author_facet | Kataria, Hardeep Shah, Navjot Kaul, Sunil C. Wadhwa, Renu Kaur, Gurcharan |
author_sort | Kataria, Hardeep |
collection | PubMed |
description | Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing activities in the water extract of Ashwagandha leaves (ASH-WEX) by examining in glioma cells. Preliminary detection for phytochemicals was performed by thin-layer chromatography. Cytotoxicity was determined using trypan blue and MTT assays. Expression level of an hsp70 family protein (mortalin), glial cell differentiation marker [glial fibrillary acidic protein (GFAP)] and neural cell adhesion molecule (NCAM) were analyzed by immunocytochemistry and immunoblotting. Anti-migratory assay was also done using wound-scratch assay. Expression levels of mortalin, GFAP and NCAM showed changes, subsequent to the treatment with ASH-WEX. The data support the existence of anti-proliferative, differentiation-inducing and anti-migratory/anti-metastasis activities in ASH-WEX that could be used as potentially safe and complimentary therapy for glioma. |
format | Text |
id | pubmed-3096473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-30964732011-06-09 Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells Kataria, Hardeep Shah, Navjot Kaul, Sunil C. Wadhwa, Renu Kaur, Gurcharan Evid Based Complement Alternat Med Original Article Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing activities in the water extract of Ashwagandha leaves (ASH-WEX) by examining in glioma cells. Preliminary detection for phytochemicals was performed by thin-layer chromatography. Cytotoxicity was determined using trypan blue and MTT assays. Expression level of an hsp70 family protein (mortalin), glial cell differentiation marker [glial fibrillary acidic protein (GFAP)] and neural cell adhesion molecule (NCAM) were analyzed by immunocytochemistry and immunoblotting. Anti-migratory assay was also done using wound-scratch assay. Expression levels of mortalin, GFAP and NCAM showed changes, subsequent to the treatment with ASH-WEX. The data support the existence of anti-proliferative, differentiation-inducing and anti-migratory/anti-metastasis activities in ASH-WEX that could be used as potentially safe and complimentary therapy for glioma. Hindawi Publishing Corporation 2011 2011-02-14 /pmc/articles/PMC3096473/ /pubmed/20007262 http://dx.doi.org/10.1093/ecam/nep188 Text en Copyright © 2011 Hardeep Kataria et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kataria, Hardeep Shah, Navjot Kaul, Sunil C. Wadhwa, Renu Kaur, Gurcharan Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells |
title | Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells |
title_full | Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells |
title_fullStr | Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells |
title_full_unstemmed | Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells |
title_short | Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells |
title_sort | water extract of ashwagandha leaves limits proliferation and migration, and induces differentiation in glioma cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096473/ https://www.ncbi.nlm.nih.gov/pubmed/20007262 http://dx.doi.org/10.1093/ecam/nep188 |
work_keys_str_mv | AT katariahardeep waterextractofashwagandhaleaveslimitsproliferationandmigrationandinducesdifferentiationingliomacells AT shahnavjot waterextractofashwagandhaleaveslimitsproliferationandmigrationandinducesdifferentiationingliomacells AT kaulsunilc waterextractofashwagandhaleaveslimitsproliferationandmigrationandinducesdifferentiationingliomacells AT wadhwarenu waterextractofashwagandhaleaveslimitsproliferationandmigrationandinducesdifferentiationingliomacells AT kaurgurcharan waterextractofashwagandhaleaveslimitsproliferationandmigrationandinducesdifferentiationingliomacells |