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Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells

Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing...

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Autores principales: Kataria, Hardeep, Shah, Navjot, Kaul, Sunil C., Wadhwa, Renu, Kaur, Gurcharan
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096473/
https://www.ncbi.nlm.nih.gov/pubmed/20007262
http://dx.doi.org/10.1093/ecam/nep188
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author Kataria, Hardeep
Shah, Navjot
Kaul, Sunil C.
Wadhwa, Renu
Kaur, Gurcharan
author_facet Kataria, Hardeep
Shah, Navjot
Kaul, Sunil C.
Wadhwa, Renu
Kaur, Gurcharan
author_sort Kataria, Hardeep
collection PubMed
description Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing activities in the water extract of Ashwagandha leaves (ASH-WEX) by examining in glioma cells. Preliminary detection for phytochemicals was performed by thin-layer chromatography. Cytotoxicity was determined using trypan blue and MTT assays. Expression level of an hsp70 family protein (mortalin), glial cell differentiation marker [glial fibrillary acidic protein (GFAP)] and neural cell adhesion molecule (NCAM) were analyzed by immunocytochemistry and immunoblotting. Anti-migratory assay was also done using wound-scratch assay. Expression levels of mortalin, GFAP and NCAM showed changes, subsequent to the treatment with ASH-WEX. The data support the existence of anti-proliferative, differentiation-inducing and anti-migratory/anti-metastasis activities in ASH-WEX that could be used as potentially safe and complimentary therapy for glioma.
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spelling pubmed-30964732011-06-09 Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells Kataria, Hardeep Shah, Navjot Kaul, Sunil C. Wadhwa, Renu Kaur, Gurcharan Evid Based Complement Alternat Med Original Article Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing activities in the water extract of Ashwagandha leaves (ASH-WEX) by examining in glioma cells. Preliminary detection for phytochemicals was performed by thin-layer chromatography. Cytotoxicity was determined using trypan blue and MTT assays. Expression level of an hsp70 family protein (mortalin), glial cell differentiation marker [glial fibrillary acidic protein (GFAP)] and neural cell adhesion molecule (NCAM) were analyzed by immunocytochemistry and immunoblotting. Anti-migratory assay was also done using wound-scratch assay. Expression levels of mortalin, GFAP and NCAM showed changes, subsequent to the treatment with ASH-WEX. The data support the existence of anti-proliferative, differentiation-inducing and anti-migratory/anti-metastasis activities in ASH-WEX that could be used as potentially safe and complimentary therapy for glioma. Hindawi Publishing Corporation 2011 2011-02-14 /pmc/articles/PMC3096473/ /pubmed/20007262 http://dx.doi.org/10.1093/ecam/nep188 Text en Copyright © 2011 Hardeep Kataria et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kataria, Hardeep
Shah, Navjot
Kaul, Sunil C.
Wadhwa, Renu
Kaur, Gurcharan
Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells
title Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells
title_full Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells
title_fullStr Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells
title_full_unstemmed Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells
title_short Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells
title_sort water extract of ashwagandha leaves limits proliferation and migration, and induces differentiation in glioma cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096473/
https://www.ncbi.nlm.nih.gov/pubmed/20007262
http://dx.doi.org/10.1093/ecam/nep188
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