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RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells
Metastatic disease is the primary cause of mortality among patients with osteogenic sarcoma (OGS). In this study, we aimed to identify the relationship of COPS3 gene expression to metastasis. Immunohistochemical staining for COPS3 was performed on 65 OGS samples (37 without and 28 with metastatic di...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3097431/ https://www.ncbi.nlm.nih.gov/pubmed/21436869 http://dx.doi.org/10.1038/cgt.2011.16 |
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author | Yan, T Tang, G Ren, T Shen, D Sun, K Liang, W Guo, W |
author_facet | Yan, T Tang, G Ren, T Shen, D Sun, K Liang, W Guo, W |
author_sort | Yan, T |
collection | PubMed |
description | Metastatic disease is the primary cause of mortality among patients with osteogenic sarcoma (OGS). In this study, we aimed to identify the relationship of COPS3 gene expression to metastasis. Immunohistochemical staining for COPS3 was performed on 65 OGS samples (37 without and 28 with metastatic disease); 18.9% (7/37) of specimens from patients with no metastasis and 57.1% (16/28) of specimens from patients with metastasis showed intense staining of COPS3. Comparison of COPS3 expression between a poorly metastatic osteosarcoma cell line (SAOS-2) and highly metastatic osteosarcoma cell line (HOS) showed stronger expression of COPS3 in HOS cells. Inhibiting COPS3 function by siRNA resulted in reduced proliferation and migration of HOS cells. Inhibition of COPS3 gene downregulated expression of the MAPK signaling pathway, which has an important role in metastasis of OGS. Our results suggested that overexpression of the COPS3 gene might have important roles in metastasis of osteosarcoma cells. |
format | Text |
id | pubmed-3097431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-30974312011-05-31 RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells Yan, T Tang, G Ren, T Shen, D Sun, K Liang, W Guo, W Cancer Gene Ther Original Article Metastatic disease is the primary cause of mortality among patients with osteogenic sarcoma (OGS). In this study, we aimed to identify the relationship of COPS3 gene expression to metastasis. Immunohistochemical staining for COPS3 was performed on 65 OGS samples (37 without and 28 with metastatic disease); 18.9% (7/37) of specimens from patients with no metastasis and 57.1% (16/28) of specimens from patients with metastasis showed intense staining of COPS3. Comparison of COPS3 expression between a poorly metastatic osteosarcoma cell line (SAOS-2) and highly metastatic osteosarcoma cell line (HOS) showed stronger expression of COPS3 in HOS cells. Inhibiting COPS3 function by siRNA resulted in reduced proliferation and migration of HOS cells. Inhibition of COPS3 gene downregulated expression of the MAPK signaling pathway, which has an important role in metastasis of OGS. Our results suggested that overexpression of the COPS3 gene might have important roles in metastasis of osteosarcoma cells. Nature Publishing Group 2011-06 2011-03-25 /pmc/articles/PMC3097431/ /pubmed/21436869 http://dx.doi.org/10.1038/cgt.2011.16 Text en Copyright © 2011 Nature America, Inc. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Original Article Yan, T Tang, G Ren, T Shen, D Sun, K Liang, W Guo, W RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells |
title | RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells |
title_full | RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells |
title_fullStr | RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells |
title_full_unstemmed | RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells |
title_short | RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells |
title_sort | rnai-mediated cops3 gene silencing inhibits metastasis of osteogenic sarcoma cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3097431/ https://www.ncbi.nlm.nih.gov/pubmed/21436869 http://dx.doi.org/10.1038/cgt.2011.16 |
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