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Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems
The physiology of the Drosophila melanogaster cardiovascular system remains poorly characterized compared with its vertebrate counterparts. Basic measures of physiological performance remain unknown. It also is unclear whether subtle physiological defects observed in the human cardiovascular system...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Company of Biologists Limited
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3097462/ https://www.ncbi.nlm.nih.gov/pubmed/21183476 http://dx.doi.org/10.1242/dmm.005231 |
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author | Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin J. Bouma, Brett E. Tearney, Guillermo J. |
author_facet | Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin J. Bouma, Brett E. Tearney, Guillermo J. |
author_sort | Choma, Michael A. |
collection | PubMed |
description | The physiology of the Drosophila melanogaster cardiovascular system remains poorly characterized compared with its vertebrate counterparts. Basic measures of physiological performance remain unknown. It also is unclear whether subtle physiological defects observed in the human cardiovascular system can be reproduced in D. melanogaster. Here we characterize the cardiovascular physiology of D. melanogaster in its pre-pupal stage by using high-speed dye angiography and optical coherence tomography. The heart has vigorous pulsatile contractions that drive intracardiac, aortic and extracellular-extravascular hemolymph flow. Several physiological measures, including weight-adjusted cardiac output, body-length-adjusted aortic velocities and intracardiac shear forces, are similar to those in the closed vertebrate cardiovascular systems, including that of humans. Extracellular-extravascular flow in the pre-pupal D. melanogaster circulation drives convection-limited fluid transport. To demonstrate homology in heart dysfunction, we showed that, at the pre-pupal stage, a troponin I mutant, held-up2 (hdp2), has impaired systolic and diastolic heart wall velocities. Impaired heart wall velocities occur in the context of a non-dilated phenotype with a mildly depressed fractional shortening. We additionally derive receiver operating characteristic curves showing that heart wall velocity is a potentially powerful discriminator of systolic heart dysfunction. Our results demonstrate physiological homology and support the use of D. melanogaster as an animal model of complex cardiovascular disease. |
format | Text |
id | pubmed-3097462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Company of Biologists Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-30974622011-06-07 Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin J. Bouma, Brett E. Tearney, Guillermo J. Dis Model Mech Research Article The physiology of the Drosophila melanogaster cardiovascular system remains poorly characterized compared with its vertebrate counterparts. Basic measures of physiological performance remain unknown. It also is unclear whether subtle physiological defects observed in the human cardiovascular system can be reproduced in D. melanogaster. Here we characterize the cardiovascular physiology of D. melanogaster in its pre-pupal stage by using high-speed dye angiography and optical coherence tomography. The heart has vigorous pulsatile contractions that drive intracardiac, aortic and extracellular-extravascular hemolymph flow. Several physiological measures, including weight-adjusted cardiac output, body-length-adjusted aortic velocities and intracardiac shear forces, are similar to those in the closed vertebrate cardiovascular systems, including that of humans. Extracellular-extravascular flow in the pre-pupal D. melanogaster circulation drives convection-limited fluid transport. To demonstrate homology in heart dysfunction, we showed that, at the pre-pupal stage, a troponin I mutant, held-up2 (hdp2), has impaired systolic and diastolic heart wall velocities. Impaired heart wall velocities occur in the context of a non-dilated phenotype with a mildly depressed fractional shortening. We additionally derive receiver operating characteristic curves showing that heart wall velocity is a potentially powerful discriminator of systolic heart dysfunction. Our results demonstrate physiological homology and support the use of D. melanogaster as an animal model of complex cardiovascular disease. The Company of Biologists Limited 2011-05 2010-12-23 /pmc/articles/PMC3097462/ /pubmed/21183476 http://dx.doi.org/10.1242/dmm.005231 Text en © 2011. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms. |
spellingShingle | Research Article Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin J. Bouma, Brett E. Tearney, Guillermo J. Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
title | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
title_full | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
title_fullStr | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
title_full_unstemmed | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
title_short | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
title_sort | physiological homology between drosophila melanogaster and vertebrate cardiovascular systems |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3097462/ https://www.ncbi.nlm.nih.gov/pubmed/21183476 http://dx.doi.org/10.1242/dmm.005231 |
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