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Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles
BACKGROUND: Enzymes belonging to acyl:CoA synthetase (ACS) superfamily activate wide variety of substrates and play major role in increasing the structural and functional diversity of various secondary metabolites in microbes and plants. However, due to the large sequence divergence within the super...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098103/ https://www.ncbi.nlm.nih.gov/pubmed/20105319 http://dx.doi.org/10.1186/1471-2105-11-57 |
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author | Khurana, Pankaj Gokhale, Rajesh S Mohanty, Debasisa |
author_facet | Khurana, Pankaj Gokhale, Rajesh S Mohanty, Debasisa |
author_sort | Khurana, Pankaj |
collection | PubMed |
description | BACKGROUND: Enzymes belonging to acyl:CoA synthetase (ACS) superfamily activate wide variety of substrates and play major role in increasing the structural and functional diversity of various secondary metabolites in microbes and plants. However, due to the large sequence divergence within the superfamily, it is difficult to predict their substrate preference by annotation transfer from the closest homolog. Therefore, a large number of ACS sequences present in public databases lack any functional annotation at the level of substrate specificity. Recently, several examples have been reported where the enzymes showing high sequence similarity to luciferases or coumarate:CoA ligases have been surprisingly found to activate fatty acyl substrates in experimental studies. In this work, we have investigated the relationship between the substrate specificity of ACS and their sequence/structural features, and developed a novel computational protocol for in silico assignment of substrate preference. RESULTS: We have used a knowledge-based approach which involves compilation of substrate specificity information for various experimentally characterized ACS and derivation of profile HMMs for each subfamily. These HMM profiles can accurately differentiate probable cognate substrates from non-cognate possibilities with high specificity (Sp) and sensitivity (Sn) (Sn = 0.91-1.0, Sp = 0.96-1.0) values. Using homologous crystal structures, we identified a limited number of contact residues crucial for substrate recognition i.e. specificity determining residues (SDRs). Patterns of SDRs from different subfamilies have been used to derive predictive rules for correlating them to substrate preference. The power of the SDR approach has been demonstrated by correct prediction of substrates for enzymes which show apparently anomalous substrate preference. Furthermore, molecular modeling of the substrates in the active site has been carried out to understand the structural basis of substrate selection. A web based prediction tool http://www.nii.res.in/pred_acs_substr.html has been developed for automated functional classification of ACS enzymes. CONCLUSIONS: We have developed a novel computational protocol for predicting substrate preference for ACS superfamily of enzymes using a limited number of SDRs. Using this approach substrate preference can be assigned to a large number of ACS enzymes present in various genomes. It can potentially help in rational design of novel proteins with altered substrate specificities. |
format | Text |
id | pubmed-3098103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30981032011-05-20 Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles Khurana, Pankaj Gokhale, Rajesh S Mohanty, Debasisa BMC Bioinformatics Research Article BACKGROUND: Enzymes belonging to acyl:CoA synthetase (ACS) superfamily activate wide variety of substrates and play major role in increasing the structural and functional diversity of various secondary metabolites in microbes and plants. However, due to the large sequence divergence within the superfamily, it is difficult to predict their substrate preference by annotation transfer from the closest homolog. Therefore, a large number of ACS sequences present in public databases lack any functional annotation at the level of substrate specificity. Recently, several examples have been reported where the enzymes showing high sequence similarity to luciferases or coumarate:CoA ligases have been surprisingly found to activate fatty acyl substrates in experimental studies. In this work, we have investigated the relationship between the substrate specificity of ACS and their sequence/structural features, and developed a novel computational protocol for in silico assignment of substrate preference. RESULTS: We have used a knowledge-based approach which involves compilation of substrate specificity information for various experimentally characterized ACS and derivation of profile HMMs for each subfamily. These HMM profiles can accurately differentiate probable cognate substrates from non-cognate possibilities with high specificity (Sp) and sensitivity (Sn) (Sn = 0.91-1.0, Sp = 0.96-1.0) values. Using homologous crystal structures, we identified a limited number of contact residues crucial for substrate recognition i.e. specificity determining residues (SDRs). Patterns of SDRs from different subfamilies have been used to derive predictive rules for correlating them to substrate preference. The power of the SDR approach has been demonstrated by correct prediction of substrates for enzymes which show apparently anomalous substrate preference. Furthermore, molecular modeling of the substrates in the active site has been carried out to understand the structural basis of substrate selection. A web based prediction tool http://www.nii.res.in/pred_acs_substr.html has been developed for automated functional classification of ACS enzymes. CONCLUSIONS: We have developed a novel computational protocol for predicting substrate preference for ACS superfamily of enzymes using a limited number of SDRs. Using this approach substrate preference can be assigned to a large number of ACS enzymes present in various genomes. It can potentially help in rational design of novel proteins with altered substrate specificities. BioMed Central 2010-01-27 /pmc/articles/PMC3098103/ /pubmed/20105319 http://dx.doi.org/10.1186/1471-2105-11-57 Text en Copyright ©2010 Khurana et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Khurana, Pankaj Gokhale, Rajesh S Mohanty, Debasisa Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles |
title | Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles |
title_full | Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles |
title_fullStr | Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles |
title_full_unstemmed | Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles |
title_short | Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles |
title_sort | genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098103/ https://www.ncbi.nlm.nih.gov/pubmed/20105319 http://dx.doi.org/10.1186/1471-2105-11-57 |
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