Cargando…
Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies
BACKGROUND: Human insulin-like growth factor-I (hIGF-I) is a growth factor which is highly resemble to insulin. It is essential for cell proliferation and has been proposed for treatment of various endocrine-associated diseases including growth hormone insensitivity syndrome and diabetes mellitus. I...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098155/ https://www.ncbi.nlm.nih.gov/pubmed/21486461 http://dx.doi.org/10.1186/1472-6750-11-37 |
_version_ | 1782203924388773888 |
---|---|
author | Cheung, Stanley CK Liu, Li-zhong Lan, Lin-lin Liu, Qiao-quan Sun, Samuel SM Chan, Juliana CN Tong, Peter CY |
author_facet | Cheung, Stanley CK Liu, Li-zhong Lan, Lin-lin Liu, Qiao-quan Sun, Samuel SM Chan, Juliana CN Tong, Peter CY |
author_sort | Cheung, Stanley CK |
collection | PubMed |
description | BACKGROUND: Human insulin-like growth factor-I (hIGF-I) is a growth factor which is highly resemble to insulin. It is essential for cell proliferation and has been proposed for treatment of various endocrine-associated diseases including growth hormone insensitivity syndrome and diabetes mellitus. In the present study, an efficient plant expression system was developed to produce biologically active recombinant hIGF-I (rhIGF-I) in transgenic rice grains. RESULTS: The plant-codon-optimized hIGF-I was introduced into rice via Agrobacterium-mediated transformation. To enhance the stability and yield of rhIGF-I, the endoplasmic reticulum-retention signal and glutelin signal peptide were used to deliver rhIGF-I to endoplasmic reticulum for stable accumulation. We found that only glutelin signal peptide could lead to successful expression of hIGF-I and one gram of hIGF-I rice grain possessed the maximum activity level equivalent to 3.2 micro molar of commercial rhIGF-I. In vitro functional analysis showed that the rice-derived rhIGF-I was effective in inducing membrane ruffling and glucose uptake on rat skeletal muscle cells. Oral meal test with rice-containing rhIGF-I acutely reduced blood glucose levels in streptozotocin-induced and Zucker diabetic rats, whereas it had no effect in normal rats. CONCLUSION: Our findings provided an alternative expression system to produce large quantities of biologically active rhIGF-I. The provision of large quantity of recombinant proteins will promote further research on the therapeutic potential of rhIGF-I. |
format | Text |
id | pubmed-3098155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30981552011-05-20 Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies Cheung, Stanley CK Liu, Li-zhong Lan, Lin-lin Liu, Qiao-quan Sun, Samuel SM Chan, Juliana CN Tong, Peter CY BMC Biotechnol Research Article BACKGROUND: Human insulin-like growth factor-I (hIGF-I) is a growth factor which is highly resemble to insulin. It is essential for cell proliferation and has been proposed for treatment of various endocrine-associated diseases including growth hormone insensitivity syndrome and diabetes mellitus. In the present study, an efficient plant expression system was developed to produce biologically active recombinant hIGF-I (rhIGF-I) in transgenic rice grains. RESULTS: The plant-codon-optimized hIGF-I was introduced into rice via Agrobacterium-mediated transformation. To enhance the stability and yield of rhIGF-I, the endoplasmic reticulum-retention signal and glutelin signal peptide were used to deliver rhIGF-I to endoplasmic reticulum for stable accumulation. We found that only glutelin signal peptide could lead to successful expression of hIGF-I and one gram of hIGF-I rice grain possessed the maximum activity level equivalent to 3.2 micro molar of commercial rhIGF-I. In vitro functional analysis showed that the rice-derived rhIGF-I was effective in inducing membrane ruffling and glucose uptake on rat skeletal muscle cells. Oral meal test with rice-containing rhIGF-I acutely reduced blood glucose levels in streptozotocin-induced and Zucker diabetic rats, whereas it had no effect in normal rats. CONCLUSION: Our findings provided an alternative expression system to produce large quantities of biologically active rhIGF-I. The provision of large quantity of recombinant proteins will promote further research on the therapeutic potential of rhIGF-I. BioMed Central 2011-04-12 /pmc/articles/PMC3098155/ /pubmed/21486461 http://dx.doi.org/10.1186/1472-6750-11-37 Text en Copyright ©2011 Cheung et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cheung, Stanley CK Liu, Li-zhong Lan, Lin-lin Liu, Qiao-quan Sun, Samuel SM Chan, Juliana CN Tong, Peter CY Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies |
title | Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies |
title_full | Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies |
title_fullStr | Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies |
title_full_unstemmed | Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies |
title_short | Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies |
title_sort | glucose lowering effect of transgenic human insulin-like growth factor-i from rice: in vitro and in vivo studies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098155/ https://www.ncbi.nlm.nih.gov/pubmed/21486461 http://dx.doi.org/10.1186/1472-6750-11-37 |
work_keys_str_mv | AT cheungstanleyck glucoseloweringeffectoftransgenichumaninsulinlikegrowthfactorifromriceinvitroandinvivostudies AT liulizhong glucoseloweringeffectoftransgenichumaninsulinlikegrowthfactorifromriceinvitroandinvivostudies AT lanlinlin glucoseloweringeffectoftransgenichumaninsulinlikegrowthfactorifromriceinvitroandinvivostudies AT liuqiaoquan glucoseloweringeffectoftransgenichumaninsulinlikegrowthfactorifromriceinvitroandinvivostudies AT sunsamuelsm glucoseloweringeffectoftransgenichumaninsulinlikegrowthfactorifromriceinvitroandinvivostudies AT chanjulianacn glucoseloweringeffectoftransgenichumaninsulinlikegrowthfactorifromriceinvitroandinvivostudies AT tongpetercy glucoseloweringeffectoftransgenichumaninsulinlikegrowthfactorifromriceinvitroandinvivostudies |