Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...

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Detalles Bibliográficos
Autores principales: Huang, Jiaqi, Zheng, Zongli, Andersson, Anders F., Engstrand, Lars, Ye, Weimin
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098247/
https://www.ncbi.nlm.nih.gov/pubmed/21625543
http://dx.doi.org/10.1371/journal.pone.0019723
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author Huang, Jiaqi
Zheng, Zongli
Andersson, Anders F.
Engstrand, Lars
Ye, Weimin
author_facet Huang, Jiaqi
Zheng, Zongli
Andersson, Anders F.
Engstrand, Lars
Ye, Weimin
author_sort Huang, Jiaqi
collection PubMed
description Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
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spelling pubmed-30982472011-05-27 Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing Huang, Jiaqi Zheng, Zongli Andersson, Anders F. Engstrand, Lars Ye, Weimin PLoS One Research Article Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample. Public Library of Science 2011-05-19 /pmc/articles/PMC3098247/ /pubmed/21625543 http://dx.doi.org/10.1371/journal.pone.0019723 Text en Huang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Huang, Jiaqi
Zheng, Zongli
Andersson, Anders F.
Engstrand, Lars
Ye, Weimin
Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
title Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
title_full Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
title_fullStr Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
title_full_unstemmed Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
title_short Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
title_sort rapid screening of complex dna samples by single-molecule amplification and sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098247/
https://www.ncbi.nlm.nih.gov/pubmed/21625543
http://dx.doi.org/10.1371/journal.pone.0019723
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