Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...

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Detalles Bibliográficos
Autores principales: Huang, Jiaqi, Zheng, Zongli, Andersson, Anders F., Engstrand, Lars, Ye, Weimin
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098247/
https://www.ncbi.nlm.nih.gov/pubmed/21625543
http://dx.doi.org/10.1371/journal.pone.0019723

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098247/
https://www.ncbi.nlm.nih.gov/pubmed/21625543
http://dx.doi.org/10.1371/journal.pone.0019723

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