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Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098247/ https://www.ncbi.nlm.nih.gov/pubmed/21625543 http://dx.doi.org/10.1371/journal.pone.0019723 |