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Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expresse...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098344/ https://www.ncbi.nlm.nih.gov/pubmed/21360135 http://dx.doi.org/10.1007/s10142-011-0213-8 |
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author | Janská, Anna Aprile, Alessio Zámečník, Jiří Cattivelli, Luigi Ovesná, Jaroslava |
author_facet | Janská, Anna Aprile, Alessio Zámečník, Jiří Cattivelli, Luigi Ovesná, Jaroslava |
author_sort | Janská, Anna |
collection | PubMed |
description | We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expressed in both the leaf and crown, or specifically in one or the other. Among the former were genes responsible for calcium and abscisic acid signalling, polyamine synthesis, late embryogenesis abundant proteins and dehydrins. In the crown, the key organ for cereal overwintering, cold treatment induced transient changes in the transcription of nucleosome assembly genes, and especially H2A and HTA11, which have been implicated in cold sensing in Arabidopsis thaliana. In the leaf, various heat-shock proteins were induced. Differences in expression pattern between the crown and leaf were frequent for genes involved in certain pathways responsible for osmolyte production (sucrose and starch, raffinose, γ-aminobutyric acid metabolism), sugar signalling (trehalose metabolism) and secondary metabolism (lignin synthesis). The action of proteins with antifreeze activity, which were markedly induced during hardening, was demonstrated by a depression in the ice nucleation temperature. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10142-011-0213-8) contains supplementary material, which is available to authorized users. |
format | Text |
id | pubmed-3098344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-30983442011-07-07 Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues Janská, Anna Aprile, Alessio Zámečník, Jiří Cattivelli, Luigi Ovesná, Jaroslava Funct Integr Genomics Original Paper We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expressed in both the leaf and crown, or specifically in one or the other. Among the former were genes responsible for calcium and abscisic acid signalling, polyamine synthesis, late embryogenesis abundant proteins and dehydrins. In the crown, the key organ for cereal overwintering, cold treatment induced transient changes in the transcription of nucleosome assembly genes, and especially H2A and HTA11, which have been implicated in cold sensing in Arabidopsis thaliana. In the leaf, various heat-shock proteins were induced. Differences in expression pattern between the crown and leaf were frequent for genes involved in certain pathways responsible for osmolyte production (sucrose and starch, raffinose, γ-aminobutyric acid metabolism), sugar signalling (trehalose metabolism) and secondary metabolism (lignin synthesis). The action of proteins with antifreeze activity, which were markedly induced during hardening, was demonstrated by a depression in the ice nucleation temperature. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10142-011-0213-8) contains supplementary material, which is available to authorized users. Springer-Verlag 2011-03-01 2011 /pmc/articles/PMC3098344/ /pubmed/21360135 http://dx.doi.org/10.1007/s10142-011-0213-8 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Paper Janská, Anna Aprile, Alessio Zámečník, Jiří Cattivelli, Luigi Ovesná, Jaroslava Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues |
title | Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues |
title_full | Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues |
title_fullStr | Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues |
title_full_unstemmed | Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues |
title_short | Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues |
title_sort | transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098344/ https://www.ncbi.nlm.nih.gov/pubmed/21360135 http://dx.doi.org/10.1007/s10142-011-0213-8 |
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