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Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues

We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expresse...

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Autores principales: Janská, Anna, Aprile, Alessio, Zámečník, Jiří, Cattivelli, Luigi, Ovesná, Jaroslava
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098344/
https://www.ncbi.nlm.nih.gov/pubmed/21360135
http://dx.doi.org/10.1007/s10142-011-0213-8
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author Janská, Anna
Aprile, Alessio
Zámečník, Jiří
Cattivelli, Luigi
Ovesná, Jaroslava
author_facet Janská, Anna
Aprile, Alessio
Zámečník, Jiří
Cattivelli, Luigi
Ovesná, Jaroslava
author_sort Janská, Anna
collection PubMed
description We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expressed in both the leaf and crown, or specifically in one or the other. Among the former were genes responsible for calcium and abscisic acid signalling, polyamine synthesis, late embryogenesis abundant proteins and dehydrins. In the crown, the key organ for cereal overwintering, cold treatment induced transient changes in the transcription of nucleosome assembly genes, and especially H2A and HTA11, which have been implicated in cold sensing in Arabidopsis thaliana. In the leaf, various heat-shock proteins were induced. Differences in expression pattern between the crown and leaf were frequent for genes involved in certain pathways responsible for osmolyte production (sucrose and starch, raffinose, γ-aminobutyric acid metabolism), sugar signalling (trehalose metabolism) and secondary metabolism (lignin synthesis). The action of proteins with antifreeze activity, which were markedly induced during hardening, was demonstrated by a depression in the ice nucleation temperature. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10142-011-0213-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-30983442011-07-07 Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues Janská, Anna Aprile, Alessio Zámečník, Jiří Cattivelli, Luigi Ovesná, Jaroslava Funct Integr Genomics Original Paper We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expressed in both the leaf and crown, or specifically in one or the other. Among the former were genes responsible for calcium and abscisic acid signalling, polyamine synthesis, late embryogenesis abundant proteins and dehydrins. In the crown, the key organ for cereal overwintering, cold treatment induced transient changes in the transcription of nucleosome assembly genes, and especially H2A and HTA11, which have been implicated in cold sensing in Arabidopsis thaliana. In the leaf, various heat-shock proteins were induced. Differences in expression pattern between the crown and leaf were frequent for genes involved in certain pathways responsible for osmolyte production (sucrose and starch, raffinose, γ-aminobutyric acid metabolism), sugar signalling (trehalose metabolism) and secondary metabolism (lignin synthesis). The action of proteins with antifreeze activity, which were markedly induced during hardening, was demonstrated by a depression in the ice nucleation temperature. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10142-011-0213-8) contains supplementary material, which is available to authorized users. Springer-Verlag 2011-03-01 2011 /pmc/articles/PMC3098344/ /pubmed/21360135 http://dx.doi.org/10.1007/s10142-011-0213-8 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Janská, Anna
Aprile, Alessio
Zámečník, Jiří
Cattivelli, Luigi
Ovesná, Jaroslava
Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
title Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
title_full Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
title_fullStr Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
title_full_unstemmed Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
title_short Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
title_sort transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098344/
https://www.ncbi.nlm.nih.gov/pubmed/21360135
http://dx.doi.org/10.1007/s10142-011-0213-8
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