Cargando…

Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows

There is significant lack of basic hematologic and immunological data in adult sows. Therefore, aim of this study was to provide respective reference intervals. 32 clinically healthy multiparous Large White sows aged 33.5 ± 9.6 months and all of them two months postpartum were included in this study...

Descripción completa

Detalles Bibliográficos
Autores principales: Sipos, Wolfgang, Duvigneau, Catharina J., Hartl, Romana T., Schwendenwein, Ilse
Formato: Texto
Lenguaje:English
Publicado: Elsevier Scientific 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098390/
https://www.ncbi.nlm.nih.gov/pubmed/21458078
http://dx.doi.org/10.1016/j.vetimm.2011.03.007
_version_ 1782203967830228992
author Sipos, Wolfgang
Duvigneau, Catharina J.
Hartl, Romana T.
Schwendenwein, Ilse
author_facet Sipos, Wolfgang
Duvigneau, Catharina J.
Hartl, Romana T.
Schwendenwein, Ilse
author_sort Sipos, Wolfgang
collection PubMed
description There is significant lack of basic hematologic and immunological data in adult sows. Therefore, aim of this study was to provide respective reference intervals. 32 clinically healthy multiparous Large White sows aged 33.5 ± 9.6 months and all of them two months postpartum were included in this study. Mean erythrocyte count was 5.5 ± 0.7 × 10(6)/μl and total leukocyte count was 12.1 ± 2.1 × 10(3)/μl. Proportion of lymphocytes was 44.7 ± 10.2% and of neutrophils 41.6 ± 11.0%. The ratio of naïve T helper (Th) cells to memory Th cells was 1:3.1 and the ratio of Th cells to cytotoxic T cells (CTLs) was 1:4.2. Proportions of regulatory T cells, NK cells, and CD21(+) B cells were lower (3.1, 2.6, and 6.0%) than those of memory Th cells ranging from 8.8 to 27.5% depending on the activation status and CTLs with 37.3%. γδ T cells were found at comparably high numbers (19.1%). Flow cytometric measurement of intracellular cytokines in PBMCs revealed marginal levels for IL-1β, IL-2, IL-4, IL-6, IL-10, and IL-12p35, but remarkable levels for TNF-α and IFN-γ. Highest mRNA levels were found for IL-1, IL-10, and TNF-α, with TNF-α showing the least inter-individual variation.
format Text
id pubmed-3098390
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Elsevier Scientific
record_format MEDLINE/PubMed
spelling pubmed-30983902011-07-12 Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows Sipos, Wolfgang Duvigneau, Catharina J. Hartl, Romana T. Schwendenwein, Ilse Vet Immunol Immunopathol Short Communication There is significant lack of basic hematologic and immunological data in adult sows. Therefore, aim of this study was to provide respective reference intervals. 32 clinically healthy multiparous Large White sows aged 33.5 ± 9.6 months and all of them two months postpartum were included in this study. Mean erythrocyte count was 5.5 ± 0.7 × 10(6)/μl and total leukocyte count was 12.1 ± 2.1 × 10(3)/μl. Proportion of lymphocytes was 44.7 ± 10.2% and of neutrophils 41.6 ± 11.0%. The ratio of naïve T helper (Th) cells to memory Th cells was 1:3.1 and the ratio of Th cells to cytotoxic T cells (CTLs) was 1:4.2. Proportions of regulatory T cells, NK cells, and CD21(+) B cells were lower (3.1, 2.6, and 6.0%) than those of memory Th cells ranging from 8.8 to 27.5% depending on the activation status and CTLs with 37.3%. γδ T cells were found at comparably high numbers (19.1%). Flow cytometric measurement of intracellular cytokines in PBMCs revealed marginal levels for IL-1β, IL-2, IL-4, IL-6, IL-10, and IL-12p35, but remarkable levels for TNF-α and IFN-γ. Highest mRNA levels were found for IL-1, IL-10, and TNF-α, with TNF-α showing the least inter-individual variation. Elsevier Scientific 2011-06-15 /pmc/articles/PMC3098390/ /pubmed/21458078 http://dx.doi.org/10.1016/j.vetimm.2011.03.007 Text en © 2011 Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Short Communication
Sipos, Wolfgang
Duvigneau, Catharina J.
Hartl, Romana T.
Schwendenwein, Ilse
Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows
title Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows
title_full Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows
title_fullStr Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows
title_full_unstemmed Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows
title_short Exploratory reference intervals on hematology and cellular immune system of multiparous Large White sows
title_sort exploratory reference intervals on hematology and cellular immune system of multiparous large white sows
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098390/
https://www.ncbi.nlm.nih.gov/pubmed/21458078
http://dx.doi.org/10.1016/j.vetimm.2011.03.007
work_keys_str_mv AT siposwolfgang exploratoryreferenceintervalsonhematologyandcellularimmunesystemofmultiparouslargewhitesows
AT duvigneaucatharinaj exploratoryreferenceintervalsonhematologyandcellularimmunesystemofmultiparouslargewhitesows
AT hartlromanat exploratoryreferenceintervalsonhematologyandcellularimmunesystemofmultiparouslargewhitesows
AT schwendenweinilse exploratoryreferenceintervalsonhematologyandcellularimmunesystemofmultiparouslargewhitesows