Cargando…
Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells
BACKGROUND: Many studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the effects of smoke exposure on allergic asthma and its asso...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098800/ https://www.ncbi.nlm.nih.gov/pubmed/21496353 http://dx.doi.org/10.1186/1465-9921-12-49 |
_version_ | 1782203995557724160 |
---|---|
author | Kim, Dae Yong Kwon, Eun Young Hong, Gwan Ui Lee, Yun Song Lee, Seung-Hyo Ro, Jai Youl |
author_facet | Kim, Dae Yong Kwon, Eun Young Hong, Gwan Ui Lee, Yun Song Lee, Seung-Hyo Ro, Jai Youl |
author_sort | Kim, Dae Yong |
collection | PubMed |
description | BACKGROUND: Many studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the effects of smoke exposure on allergic asthma and its association with mast cells. METHODS: BALB/c mice were sensitized and challenged by OVA to induce asthma, and bone marrow-derived mast cells (BMMCs) were stimulated with antigen/antibody reaction. Mice or BMMCs were exposed to cigarette smoke or CSE solution for 1 mo or 6 h, respectively. The recruitment of inflammatory cells into BAL fluid or lung tissues was determined by Diff-Quik or H&E staining, collagen deposition by Sircol assay, penh values by a whole-body plethysmography, co-localization of tryptase and Smad3 by immunohistochemistry, IgE and TGF-β level by ELISA, expressions of Smads proteins, activities of signaling molecules, or TGF-β mRNA by immunoblotting and RT-PCR. RESULTS: Cigarette smoke enhanced OVA-specific IgE levels, penh values, recruitment of inflammatory cells including mast cells, expressions of smad family, TGF-β mRNA and proteins, and cytokines, phosphorylations of Smad2 and 3, and MAP kinases, co-localization of tryptase and Smad3, and collagen deposition more than those of BAL cells and lung tissues of OVA-induced allergic mice. CSE solution pretreatment enhanced expressions of TGF-β, Smad3, activities of MAP kinases, NF-κB/AP-1 or PAI-1 more than those of activated-BMMCs. CONCLUSIONS: The data suggest that smoke exposure enhances antigen-induced mast cell activation via TGF-β/Smad signaling pathways in mouse allergic asthma, and that it exacerbates airway inflammation and remodeling. |
format | Text |
id | pubmed-3098800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30988002011-05-21 Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells Kim, Dae Yong Kwon, Eun Young Hong, Gwan Ui Lee, Yun Song Lee, Seung-Hyo Ro, Jai Youl Respir Res Research BACKGROUND: Many studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the effects of smoke exposure on allergic asthma and its association with mast cells. METHODS: BALB/c mice were sensitized and challenged by OVA to induce asthma, and bone marrow-derived mast cells (BMMCs) were stimulated with antigen/antibody reaction. Mice or BMMCs were exposed to cigarette smoke or CSE solution for 1 mo or 6 h, respectively. The recruitment of inflammatory cells into BAL fluid or lung tissues was determined by Diff-Quik or H&E staining, collagen deposition by Sircol assay, penh values by a whole-body plethysmography, co-localization of tryptase and Smad3 by immunohistochemistry, IgE and TGF-β level by ELISA, expressions of Smads proteins, activities of signaling molecules, or TGF-β mRNA by immunoblotting and RT-PCR. RESULTS: Cigarette smoke enhanced OVA-specific IgE levels, penh values, recruitment of inflammatory cells including mast cells, expressions of smad family, TGF-β mRNA and proteins, and cytokines, phosphorylations of Smad2 and 3, and MAP kinases, co-localization of tryptase and Smad3, and collagen deposition more than those of BAL cells and lung tissues of OVA-induced allergic mice. CSE solution pretreatment enhanced expressions of TGF-β, Smad3, activities of MAP kinases, NF-κB/AP-1 or PAI-1 more than those of activated-BMMCs. CONCLUSIONS: The data suggest that smoke exposure enhances antigen-induced mast cell activation via TGF-β/Smad signaling pathways in mouse allergic asthma, and that it exacerbates airway inflammation and remodeling. BioMed Central 2011 2011-04-18 /pmc/articles/PMC3098800/ /pubmed/21496353 http://dx.doi.org/10.1186/1465-9921-12-49 Text en Copyright ©2011 Kim et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Kim, Dae Yong Kwon, Eun Young Hong, Gwan Ui Lee, Yun Song Lee, Seung-Hyo Ro, Jai Youl Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
title | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
title_full | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
title_fullStr | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
title_full_unstemmed | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
title_short | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
title_sort | cigarette smoke exacerbates mouse allergic asthma through smad proteins expressed in mast cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098800/ https://www.ncbi.nlm.nih.gov/pubmed/21496353 http://dx.doi.org/10.1186/1465-9921-12-49 |
work_keys_str_mv | AT kimdaeyong cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT kwoneunyoung cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT honggwanui cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT leeyunsong cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT leeseunghyo cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT rojaiyoul cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells |