Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol

Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losse...

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Detalles Bibliográficos
Autores principales: Wilson, Eli Adjélé, Ennahar, Saïd, Zhao, Minjie, Bergaentzle, Martine, Marchioni, Eric, Bindler, Françoise
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Full Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098979/
https://www.ncbi.nlm.nih.gov/pubmed/21765536
http://dx.doi.org/10.1007/s10337-010-1878-1
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author Wilson, Eli Adjélé
Ennahar, Saïd
Zhao, Minjie
Bergaentzle, Martine
Marchioni, Eric
Bindler, Françoise
author_facet Wilson, Eli Adjélé
Ennahar, Saïd
Zhao, Minjie
Bergaentzle, Martine
Marchioni, Eric
Bindler, Françoise
author_sort Wilson, Eli Adjélé
collection PubMed
description Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5–32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 μg g(−1) fresh weight, respectively.
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spelling pubmed-30989792011-07-14 Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol Wilson, Eli Adjélé Ennahar, Saïd Zhao, Minjie Bergaentzle, Martine Marchioni, Eric Bindler, Françoise Chromatographia Full Short Communication Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5–32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 μg g(−1) fresh weight, respectively. Springer-Verlag 2011-01-08 2011 /pmc/articles/PMC3098979/ /pubmed/21765536 http://dx.doi.org/10.1007/s10337-010-1878-1 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Full Short Communication
Wilson, Eli Adjélé
Ennahar, Saïd
Zhao, Minjie
Bergaentzle, Martine
Marchioni, Eric
Bindler, Françoise
Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
title Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
title_full Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
title_fullStr Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
title_full_unstemmed Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
title_short Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
title_sort simultaneous determination of various isothiocyanates by rp-lc following precolumn derivatization with mercaptoethanol
topic Full Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098979/
https://www.ncbi.nlm.nih.gov/pubmed/21765536
http://dx.doi.org/10.1007/s10337-010-1878-1
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