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HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland

Human papillomaviruses (HPVs) are a very complex group of pathogenic viruses, with more than 80 types, causing human infection. Given the prevalence of HPV infection and its relationship with the development of cervical and many other cancers, HPV vaccine development has been a major public health i...

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Autores principales: Durzyńska, Julia, Pacholska-Bogalska, Joanna, Kaczmarek, Maria, Hanć, Tomasz, Durda, Magdalena, Skrzypczak, Magdalena, Goździcka-Józefiak, Anna
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098990/
https://www.ncbi.nlm.nih.gov/pubmed/21107606
http://dx.doi.org/10.1007/s00431-010-1345-x
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author Durzyńska, Julia
Pacholska-Bogalska, Joanna
Kaczmarek, Maria
Hanć, Tomasz
Durda, Magdalena
Skrzypczak, Magdalena
Goździcka-Józefiak, Anna
author_facet Durzyńska, Julia
Pacholska-Bogalska, Joanna
Kaczmarek, Maria
Hanć, Tomasz
Durda, Magdalena
Skrzypczak, Magdalena
Goździcka-Józefiak, Anna
author_sort Durzyńska, Julia
collection PubMed
description Human papillomaviruses (HPVs) are a very complex group of pathogenic viruses, with more than 80 types, causing human infection. Given the prevalence of HPV infection and its relationship with the development of cervical and many other cancers, HPV vaccine development has been a major public health initiative worldwide in the last decade. The aim of the presented study was to identify HPV DNA by MY-PCR in 4,150 school children and adolescents, aged 10–18 years in the Wielkopolska region, Poland. All individuals were asked to fill in extensive questionnaires; further normal, oral squamous cells were collected from each pupil. Cellular DNA was isolated and used as a MY-PCR template to estimate the incidence of HPV-active infection. Forty five subjects (1.08% of the sample) were carriers of oropharyngeal HPVs. HPV status and variables of interest, such as age, gender, socioeconomical status, and risk factors (smoking and sexual intercourse history, alcohol consumption) were not correlated. The presence of HPVs in the oral cavity was cumulated in several schools of the region. DNA sequencing of MY-PCR products revealed only four HPV genotypes. The most frequent genotype was HPV11 (38/45 HPV-positive cases), while other more rare genotypes were HPV6 (3/45), HPV12 (3/45), and HPV57 (1/45). Conclusion: Our findings presented herein, reveal a relatively low prevalance of oropharyngeal HPVs in Polish adolescents and fill an important gap in the knowledge of oral HPV infections of children above 10 years and adolescents.
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spelling pubmed-30989902011-07-14 HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland Durzyńska, Julia Pacholska-Bogalska, Joanna Kaczmarek, Maria Hanć, Tomasz Durda, Magdalena Skrzypczak, Magdalena Goździcka-Józefiak, Anna Eur J Pediatr Original Paper Human papillomaviruses (HPVs) are a very complex group of pathogenic viruses, with more than 80 types, causing human infection. Given the prevalence of HPV infection and its relationship with the development of cervical and many other cancers, HPV vaccine development has been a major public health initiative worldwide in the last decade. The aim of the presented study was to identify HPV DNA by MY-PCR in 4,150 school children and adolescents, aged 10–18 years in the Wielkopolska region, Poland. All individuals were asked to fill in extensive questionnaires; further normal, oral squamous cells were collected from each pupil. Cellular DNA was isolated and used as a MY-PCR template to estimate the incidence of HPV-active infection. Forty five subjects (1.08% of the sample) were carriers of oropharyngeal HPVs. HPV status and variables of interest, such as age, gender, socioeconomical status, and risk factors (smoking and sexual intercourse history, alcohol consumption) were not correlated. The presence of HPVs in the oral cavity was cumulated in several schools of the region. DNA sequencing of MY-PCR products revealed only four HPV genotypes. The most frequent genotype was HPV11 (38/45 HPV-positive cases), while other more rare genotypes were HPV6 (3/45), HPV12 (3/45), and HPV57 (1/45). Conclusion: Our findings presented herein, reveal a relatively low prevalance of oropharyngeal HPVs in Polish adolescents and fill an important gap in the knowledge of oral HPV infections of children above 10 years and adolescents. Springer-Verlag 2010-11-24 2011 /pmc/articles/PMC3098990/ /pubmed/21107606 http://dx.doi.org/10.1007/s00431-010-1345-x Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Durzyńska, Julia
Pacholska-Bogalska, Joanna
Kaczmarek, Maria
Hanć, Tomasz
Durda, Magdalena
Skrzypczak, Magdalena
Goździcka-Józefiak, Anna
HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland
title HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland
title_full HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland
title_fullStr HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland
title_full_unstemmed HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland
title_short HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland
title_sort hpv genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in poland
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098990/
https://www.ncbi.nlm.nih.gov/pubmed/21107606
http://dx.doi.org/10.1007/s00431-010-1345-x
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