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Non-association of IL-12 +1188 and IFN-γ +874 Polymorphisms with Cytokines Serum Level in Occult HBV Infected Patients

BACKGROUND/AIM: Occult hepatitis B infection (OBI) is identified as a form of hepatitis in which despite the absence of detectable HBsAg, HBV-DNA is observed in peripheral blood of patients. The main aim of this study has been to investigate the association between polymorphisms in +874 of IFN-γ and...

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Detalles Bibliográficos
Autores principales: Arababadi, Mohammad K., Pourfathollah, Ali A., Jafarzadeh, Abdollah, Hassanshahi, Gholamhossein, Daneshmandi, Saeed, Shamsizadeh, Ali, Kennedy, Derek
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3099077/
https://www.ncbi.nlm.nih.gov/pubmed/21196650
http://dx.doi.org/10.4103/1319-3767.74461
Descripción
Sumario:BACKGROUND/AIM: Occult hepatitis B infection (OBI) is identified as a form of hepatitis in which despite the absence of detectable HBsAg, HBV-DNA is observed in peripheral blood of patients. The main aim of this study has been to investigate the association between polymorphisms in +874 of IFN-γ and +1188 of IL-12 with their serum level in patients suffering from OBI. MATERIALS AND METHODS: In this experimental study, plasma samples of 3700 blood donors were tested for the presence of hepatitis B surface antigen (HBsAg) and anti-HBc by ELISA. The HBsAg(-)/anti-HBc(+) samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples were assigned as OBI cases and ARMS-PCR techniques were performed to examine the two known polymorphisms within IL-12 and IFN-γ. In addition, the serum levels of IL-12 and IFN-γ were also determined by ELISA. RESULTS: Results of this study demonstrated that, 352 (9.5%) out of 3700 blood samples were HBsAg(-)/anti-HBc(+) and HBV-DNA was detected in 57/352 (16.1%) of HBsAg(-)/anti-HBc(+) samples. Our results showed that groups showed significant difference in CC allele of +1188 region of IL-12 and no difference was observed in the other evaluated genes. Our results also showed that the alleles of +1188 region of IL-12 and alleles of +874 of IFN-γ were also not associated with serum level of cytokines. CONCLUSION: According to the results of this study, it may be concluded that the polymorphisms in +1188 region of IL-12 and +874 region of IFN-γ would not affect the expression of both cytokines at serum level in OBI patients.