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Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis
BACKGROUND & OBJECTIVES: Acanthamoeba keratitis (AK) is a painful and vision-threatening ocular infection. The differentiation of Acanthamoeba at the species and subspecies level is complicated. Nearly all the AK isolates have been shown to belong to T4 genotype when analysed by ribosomal RNA ge...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Medknow Publications
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100151/ https://www.ncbi.nlm.nih.gov/pubmed/21321424 |
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author | Prashanth, K. Pasricha, Gunisha Sharma, Savitri |
author_facet | Prashanth, K. Pasricha, Gunisha Sharma, Savitri |
author_sort | Prashanth, K. |
collection | PubMed |
description | BACKGROUND & OBJECTIVES: Acanthamoeba keratitis (AK) is a painful and vision-threatening ocular infection. The differentiation of Acanthamoeba at the species and subspecies level is complicated. Nearly all the AK isolates have been shown to belong to T4 genotype when analysed by ribosomal RNA gene sequences and there is no universally acceptable method for differentiation of different subtypes of T4. The purpose of this study was to attempt further discrimination of T4 genotypes. METHODS: In the present investigation, 15 Acanthamoeba isolates obtained from cornea of keratitis patients were subjected to fluorescence amplified fragment length polymorphism (FAFLP) genotyping to differentiate T4 subtypes. RESULTS: FAFLP profiles showed five distinct clusters (I to V) within T4 clonal complex which clearly depicted genetic differences among the isolates of T4 sequence type of Acanthamoeba. INTERPRETATION & CONCLUSIONS: Our study demonstrated the usefulness of FAFLP for reliable differentiation of T4 clonal complex of Acanthamoeba. |
format | Text |
id | pubmed-3100151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-31001512011-06-08 Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis Prashanth, K. Pasricha, Gunisha Sharma, Savitri Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Acanthamoeba keratitis (AK) is a painful and vision-threatening ocular infection. The differentiation of Acanthamoeba at the species and subspecies level is complicated. Nearly all the AK isolates have been shown to belong to T4 genotype when analysed by ribosomal RNA gene sequences and there is no universally acceptable method for differentiation of different subtypes of T4. The purpose of this study was to attempt further discrimination of T4 genotypes. METHODS: In the present investigation, 15 Acanthamoeba isolates obtained from cornea of keratitis patients were subjected to fluorescence amplified fragment length polymorphism (FAFLP) genotyping to differentiate T4 subtypes. RESULTS: FAFLP profiles showed five distinct clusters (I to V) within T4 clonal complex which clearly depicted genetic differences among the isolates of T4 sequence type of Acanthamoeba. INTERPRETATION & CONCLUSIONS: Our study demonstrated the usefulness of FAFLP for reliable differentiation of T4 clonal complex of Acanthamoeba. Medknow Publications 2011-01 /pmc/articles/PMC3100151/ /pubmed/21321424 Text en © The Indian Journal of Medical Research http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Prashanth, K. Pasricha, Gunisha Sharma, Savitri Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis |
title | Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis |
title_full | Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis |
title_fullStr | Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis |
title_full_unstemmed | Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis |
title_short | Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis |
title_sort | fluorescence amplified fragment length polymorphism for subtyping of genotypes of acanthamoeba isolated from patients with keratitis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100151/ https://www.ncbi.nlm.nih.gov/pubmed/21321424 |
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