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Dendritic cell populations in patients with self-reported food hypersensitivity

Self-reported hypersensitivity to food is a common condition and many of these patients have indications of intestinal immune activation. Dendritic cells (DCs) are recognized as the most potent antigen-presenting cells involved in both initiating immune responses and maintaining tolerance. The aims...

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Detalles Bibliográficos
Autores principales: Lied, Gülen A, Vogelsang, Petra, Berstad, Arnold, Appel, Silke
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100221/
https://www.ncbi.nlm.nih.gov/pubmed/21625415
http://dx.doi.org/10.2147/IJGM.S17655
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author Lied, Gülen A
Vogelsang, Petra
Berstad, Arnold
Appel, Silke
author_facet Lied, Gülen A
Vogelsang, Petra
Berstad, Arnold
Appel, Silke
author_sort Lied, Gülen A
collection PubMed
description Self-reported hypersensitivity to food is a common condition and many of these patients have indications of intestinal immune activation. Dendritic cells (DCs) are recognized as the most potent antigen-presenting cells involved in both initiating immune responses and maintaining tolerance. The aims of this study were to evaluate the DC populations with their phenotype and T cell stimulatory capacity in patients with food hypersensitivity and to study its relationship with atopic disease. Blood samples from 10 patients with self-reported food hypersensitivity, divided into atopic and nonatopic subgroups, and 10 gender- and age-matched healthy controls were analyzed by flow cytometry using the Miltenyi Blood Dendritic cells kit. Monocyte-derived DCs (moDCs) were evaluated concerning their phenotype and T cell stimulatory capacity. DC populations and cell surface markers were not significantly different between patients and healthy controls, but moDCs from atopic patients expressed significantly more CD38 compared to moDCs from nonatopic patients. Moreover, lipopolysaccharide stimulated moDCs from atopic patients produced significantly more interleukin-10 compared to nonatopic patients. CD38 expression was correlated to total serum immunoglobulin E levels. These findings support the notion of immune activation in some patients with self-reported food hypersensitivity. They need to be confirmed in a larger cohort.
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spelling pubmed-31002212011-05-27 Dendritic cell populations in patients with self-reported food hypersensitivity Lied, Gülen A Vogelsang, Petra Berstad, Arnold Appel, Silke Int J Gen Med Original Research Self-reported hypersensitivity to food is a common condition and many of these patients have indications of intestinal immune activation. Dendritic cells (DCs) are recognized as the most potent antigen-presenting cells involved in both initiating immune responses and maintaining tolerance. The aims of this study were to evaluate the DC populations with their phenotype and T cell stimulatory capacity in patients with food hypersensitivity and to study its relationship with atopic disease. Blood samples from 10 patients with self-reported food hypersensitivity, divided into atopic and nonatopic subgroups, and 10 gender- and age-matched healthy controls were analyzed by flow cytometry using the Miltenyi Blood Dendritic cells kit. Monocyte-derived DCs (moDCs) were evaluated concerning their phenotype and T cell stimulatory capacity. DC populations and cell surface markers were not significantly different between patients and healthy controls, but moDCs from atopic patients expressed significantly more CD38 compared to moDCs from nonatopic patients. Moreover, lipopolysaccharide stimulated moDCs from atopic patients produced significantly more interleukin-10 compared to nonatopic patients. CD38 expression was correlated to total serum immunoglobulin E levels. These findings support the notion of immune activation in some patients with self-reported food hypersensitivity. They need to be confirmed in a larger cohort. Dove Medical Press 2011-05-15 /pmc/articles/PMC3100221/ /pubmed/21625415 http://dx.doi.org/10.2147/IJGM.S17655 Text en © 2011 Lied et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Lied, Gülen A
Vogelsang, Petra
Berstad, Arnold
Appel, Silke
Dendritic cell populations in patients with self-reported food hypersensitivity
title Dendritic cell populations in patients with self-reported food hypersensitivity
title_full Dendritic cell populations in patients with self-reported food hypersensitivity
title_fullStr Dendritic cell populations in patients with self-reported food hypersensitivity
title_full_unstemmed Dendritic cell populations in patients with self-reported food hypersensitivity
title_short Dendritic cell populations in patients with self-reported food hypersensitivity
title_sort dendritic cell populations in patients with self-reported food hypersensitivity
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100221/
https://www.ncbi.nlm.nih.gov/pubmed/21625415
http://dx.doi.org/10.2147/IJGM.S17655
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