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Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen
BACKGROUND: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. METHODS: Splenocytes from...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Association of Immunologists
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100520/ https://www.ncbi.nlm.nih.gov/pubmed/21637387 http://dx.doi.org/10.4110/in.2011.11.2.100 |
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author | Jeon, Ji Yeong Jung, Kyung Min Chang, Jun Choi, Eun Young |
author_facet | Jeon, Ji Yeong Jung, Kyung Min Chang, Jun Choi, Eun Young |
author_sort | Jeon, Ji Yeong |
collection | PubMed |
description | BACKGROUND: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. METHODS: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/H-2K(b) and analyzed for TCR sequences. RESULTS: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/H-2K(b) with t(1/2) values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were Vα12D-3-01+Jα11-01 and Vβ12-1-01+Dβ1-01+J2-7-01 for clone #14, Vα13D-1-02+Jα34-02 and Vβ13-1-02+Dβ2-01+Jβ2-7-01 for clone #15, and Vα16D+Jα45-01 and Vβ12-1-01+Dβ1-01+Jβ2-5-01 for clone #23. CONCLUSION: The results will be useful for modeling GVL and generation TCR transgenic mouse. |
format | Text |
id | pubmed-3100520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Association of Immunologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-31005202011-06-02 Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen Jeon, Ji Yeong Jung, Kyung Min Chang, Jun Choi, Eun Young Immune Netw Original Article BACKGROUND: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. METHODS: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/H-2K(b) and analyzed for TCR sequences. RESULTS: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/H-2K(b) with t(1/2) values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were Vα12D-3-01+Jα11-01 and Vβ12-1-01+Dβ1-01+J2-7-01 for clone #14, Vα13D-1-02+Jα34-02 and Vβ13-1-02+Dβ2-01+Jβ2-7-01 for clone #15, and Vα16D+Jα45-01 and Vβ12-1-01+Dβ1-01+Jβ2-5-01 for clone #23. CONCLUSION: The results will be useful for modeling GVL and generation TCR transgenic mouse. The Korean Association of Immunologists 2011-04 2011-04-30 /pmc/articles/PMC3100520/ /pubmed/21637387 http://dx.doi.org/10.4110/in.2011.11.2.100 Text en Copyright © 2011 The Korean Association of Immunologists http://creativecommons.org/licenses/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jeon, Ji Yeong Jung, Kyung Min Chang, Jun Choi, Eun Young Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen |
title | Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen |
title_full | Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen |
title_fullStr | Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen |
title_full_unstemmed | Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen |
title_short | Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen |
title_sort | characterization of ctl clones specific for single antigen, h60 minor histocompatibility antigen |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100520/ https://www.ncbi.nlm.nih.gov/pubmed/21637387 http://dx.doi.org/10.4110/in.2011.11.2.100 |
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