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Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol
The replacement of disease hepatocytes and the stimulation of endogenous or exogenous regeneration by human mesenchymal stem cells (MSCs) are promising candidates for liver-directed cell therapy. In this study, we isolated MSCs from adult bone marrow by plastic adhesion and induced differentiation w...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100820/ https://www.ncbi.nlm.nih.gov/pubmed/21614181 http://dx.doi.org/10.3390/ijms11124905 |
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author | Leelawat, Kawin Narong, Siriluck Chaijan, Suthidarak Sa-ngiamsuntorn, Khanit Disthabanchong, Sinee Wongkajornsilp, Adisak Hongeng, Suradej |
author_facet | Leelawat, Kawin Narong, Siriluck Chaijan, Suthidarak Sa-ngiamsuntorn, Khanit Disthabanchong, Sinee Wongkajornsilp, Adisak Hongeng, Suradej |
author_sort | Leelawat, Kawin |
collection | PubMed |
description | The replacement of disease hepatocytes and the stimulation of endogenous or exogenous regeneration by human mesenchymal stem cells (MSCs) are promising candidates for liver-directed cell therapy. In this study, we isolated MSCs from adult bone marrow by plastic adhesion and induced differentiation with a liver differentiation protocol. Western blot analyses were used to assess the expression of liver-specific markers. Next, MSC-specific proteins were analyzed with two-dimensional (2D) gel electrophoresis and peptide mass fingerprinting matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF)-mass spectrometry (MS). To confirm the results from the proteomic study, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses were performed. We demonstrated that MSCs treated with the liver differentiation protocol expressed significantly more albumin, CK19 and CK20, than did undifferentiated cells. In addition the results of proteomic study demonstrated increases expression of FEM1B, PSMC2 and disulfide-isomerase A3 in MSCs treated with the liver differentiation protocol. These results from proteomic profiling will not only provide insight into the global responses of MSCs to hepatocyte differentiation, but will also lead to in-depth studies on the mechanisms of proteomic changes in MSCs. |
format | Text |
id | pubmed-3100820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-31008202011-05-25 Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol Leelawat, Kawin Narong, Siriluck Chaijan, Suthidarak Sa-ngiamsuntorn, Khanit Disthabanchong, Sinee Wongkajornsilp, Adisak Hongeng, Suradej Int J Mol Sci Article The replacement of disease hepatocytes and the stimulation of endogenous or exogenous regeneration by human mesenchymal stem cells (MSCs) are promising candidates for liver-directed cell therapy. In this study, we isolated MSCs from adult bone marrow by plastic adhesion and induced differentiation with a liver differentiation protocol. Western blot analyses were used to assess the expression of liver-specific markers. Next, MSC-specific proteins were analyzed with two-dimensional (2D) gel electrophoresis and peptide mass fingerprinting matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF)-mass spectrometry (MS). To confirm the results from the proteomic study, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses were performed. We demonstrated that MSCs treated with the liver differentiation protocol expressed significantly more albumin, CK19 and CK20, than did undifferentiated cells. In addition the results of proteomic study demonstrated increases expression of FEM1B, PSMC2 and disulfide-isomerase A3 in MSCs treated with the liver differentiation protocol. These results from proteomic profiling will not only provide insight into the global responses of MSCs to hepatocyte differentiation, but will also lead to in-depth studies on the mechanisms of proteomic changes in MSCs. Molecular Diversity Preservation International (MDPI) 2010-11-30 /pmc/articles/PMC3100820/ /pubmed/21614181 http://dx.doi.org/10.3390/ijms11124905 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Leelawat, Kawin Narong, Siriluck Chaijan, Suthidarak Sa-ngiamsuntorn, Khanit Disthabanchong, Sinee Wongkajornsilp, Adisak Hongeng, Suradej Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol |
title | Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol |
title_full | Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol |
title_fullStr | Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol |
title_full_unstemmed | Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol |
title_short | Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol |
title_sort | proteomic profiles of mesenchymal stem cells induced by a liver differentiation protocol |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100820/ https://www.ncbi.nlm.nih.gov/pubmed/21614181 http://dx.doi.org/10.3390/ijms11124905 |
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