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Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells
The present study was designed to investigate the question of whether or not astaxanthin improves stem cell potency via an increase in proliferation of neural progenitor cells (NPCs). Treatment with astaxanthin significantly increased proliferation and colony formation of NPCs. For identification of...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100832/ https://www.ncbi.nlm.nih.gov/pubmed/21614195 http://dx.doi.org/10.3390/ijms11125109 |
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author | Kim, Jeong-Hwan Nam, Soo-Wan Kim, Byung-Woo Choi, Woobong Lee, Jong-Hwan Kim, Wun-Jae Choi, Yung-Hyun |
author_facet | Kim, Jeong-Hwan Nam, Soo-Wan Kim, Byung-Woo Choi, Woobong Lee, Jong-Hwan Kim, Wun-Jae Choi, Yung-Hyun |
author_sort | Kim, Jeong-Hwan |
collection | PubMed |
description | The present study was designed to investigate the question of whether or not astaxanthin improves stem cell potency via an increase in proliferation of neural progenitor cells (NPCs). Treatment with astaxanthin significantly increased proliferation and colony formation of NPCs. For identification of possible activated signaling molecules involved in active cell proliferation occurring after astaxanthin treatment, total protein levels of several proliferation-related proteins, and expression levels of proliferation-related transcription factors, were assessed in NPCs. In Western blot analysis, astaxanthin induced significant activation of phosphatidylinositol 3-kinase (PI3K) and its downstream mediators in a time-dependent manner. Results of RT-PCR analysis showed upregulation of proliferation-related transcription factors and stemness genes. To estimate the relevance of PI3K and mitogen-activated protein, or extracellular signal-regulated kinase kinase (MEK) signaling pathways in cell growth of astaxanthin-treated NPCs, inhibition assays were performed with LY294002, a specific inhibitor of PI3K, and PD98059, a specific inhibitor of MEK, respectively. These results clearly showed that astaxanthin induces proliferation of NPCs via activation of the PI3K and MEK signaling pathways and improves stem cell potency via stemness acting signals. |
format | Text |
id | pubmed-3100832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-31008322011-05-25 Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells Kim, Jeong-Hwan Nam, Soo-Wan Kim, Byung-Woo Choi, Woobong Lee, Jong-Hwan Kim, Wun-Jae Choi, Yung-Hyun Int J Mol Sci Article The present study was designed to investigate the question of whether or not astaxanthin improves stem cell potency via an increase in proliferation of neural progenitor cells (NPCs). Treatment with astaxanthin significantly increased proliferation and colony formation of NPCs. For identification of possible activated signaling molecules involved in active cell proliferation occurring after astaxanthin treatment, total protein levels of several proliferation-related proteins, and expression levels of proliferation-related transcription factors, were assessed in NPCs. In Western blot analysis, astaxanthin induced significant activation of phosphatidylinositol 3-kinase (PI3K) and its downstream mediators in a time-dependent manner. Results of RT-PCR analysis showed upregulation of proliferation-related transcription factors and stemness genes. To estimate the relevance of PI3K and mitogen-activated protein, or extracellular signal-regulated kinase kinase (MEK) signaling pathways in cell growth of astaxanthin-treated NPCs, inhibition assays were performed with LY294002, a specific inhibitor of PI3K, and PD98059, a specific inhibitor of MEK, respectively. These results clearly showed that astaxanthin induces proliferation of NPCs via activation of the PI3K and MEK signaling pathways and improves stem cell potency via stemness acting signals. Molecular Diversity Preservation International (MDPI) 2010-12-09 /pmc/articles/PMC3100832/ /pubmed/21614195 http://dx.doi.org/10.3390/ijms11125109 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kim, Jeong-Hwan Nam, Soo-Wan Kim, Byung-Woo Choi, Woobong Lee, Jong-Hwan Kim, Wun-Jae Choi, Yung-Hyun Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells |
title | Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells |
title_full | Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells |
title_fullStr | Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells |
title_full_unstemmed | Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells |
title_short | Astaxanthin Improves Stem Cell Potency via an Increase in the Proliferation of Neural Progenitor Cells |
title_sort | astaxanthin improves stem cell potency via an increase in the proliferation of neural progenitor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100832/ https://www.ncbi.nlm.nih.gov/pubmed/21614195 http://dx.doi.org/10.3390/ijms11125109 |
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