A Simple and Rapid Method for DNA Isolation from Xylophagous Insects

Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (...

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Detalles Bibliográficos
Autores principales: Calderón-Cortés, Nancy, Quesada, Mauricio, Cano-Camacho, Horacio, Zavala-Páramo, Guadalupe
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100841/
https://www.ncbi.nlm.nih.gov/pubmed/21614191
http://dx.doi.org/10.3390/ijms11125056
Descripción
Sumario:Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses.

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